Book Chapter10.1016/BS.AAMBS.2021.03.003
Lipid production by oleaginous yeasts.
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TL;DR: In this paper, the authors highlight the prominent oleaginous yeast genera, emphasizing their oleagineous characteristics, in conjunction with diverse other features such as cheap carbon source utilization, withstanding the effect of inhibitory compounds, commercially favorable fatty acid composition, and supporting their future development as economically viable lipid feedstock.
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Abstract: Microbial lipid production has been studied extensively for years; however, lipid metabolic engineering in many of the extraordinarily high lipid-accumulating yeasts was impeded by inadequate understanding of the metabolic pathways including regulatory mechanisms defining their oleaginicity and the limited genetic tools available. The aim of this review is to highlight the prominent oleaginous yeast genera, emphasizing their oleaginous characteristics, in conjunction with diverse other features such as cheap carbon source utilization, withstanding the effect of inhibitory compounds, commercially favorable fatty acid composition—all supporting their future development as economically viable lipid feedstock. The unique aspects of metabolism attributing to their oleaginicity are accentuated in the pretext of outlining the various strategies successfully implemented to improve the production of lipid and lipid-derived metabolites. A large number of in silico data generated on the lipid accumulation in certain oleaginous yeasts have been carefully curated, as suggestive evidences in line with the exceptional oleaginicity of these organisms. The different genetic elements developed in these yeasts to execute such strategies have been scrupulously inspected, underlining the major types of newly-found and synthetically constructed promoters, transcription terminators, and selection markers. Additionally, there is a plethora of advanced genetic toolboxes and techniques described, which have been successfully used in oleaginous yeasts in the recent years, promoting homologous recombination, genome editing, DNA assembly, and transformation at remarkable efficiencies. They can accelerate and effectively guide the rational designing of system-wide metabolic engineering approaches pinpointing the key targets for developing industrially suitable yeast strains.
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Citations
Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review
Sameh S. Ali,Rania Al-Tohamy,Tarek Mohamed,Yehia A.-G. Mahmoud,Héctor A. Ruiz,Lushan Sun,Jianzhong Sun +6 more
TL;DR: In this article , the authors used robust oleaginous yeast as a cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation, where the highest percentage of lipid accumulation can be achieved as high as 47.25% by Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system.
Combination of simultaneous saccharification and fermentation of corn stover with consolidated bioprocessing of cassava starch enhances lipid production by the amylolytic oleaginous yeast Lipomyces starkeyi.
TL;DR: In this article , a combination of simultaneous saccharification and fermentation of corn stover with consolidated bioprocessing (CBP) of cassava starch by Lipomyces starkeyi was firstly developed as a novel strategy for lipid production.
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Potential of Rhodosporidium toruloides for Fatty Acids Production Using Lignocellulose Biomass.
TL;DR: This review provides useful information on the research working to develop processes for lignocellulose biomass using oleaginous yeast utilizing Rhodosporidium spp.
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Recent advances in genetic technology development of oleaginous yeasts
TL;DR: Recent progress in genetic technology with regard to oleaginous yeasts is reviewed, with a special focus on transformation methods and genome editing tools, discussing the effects of some important genetic parts.
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Production of single cell oil by two novel nonconventional yeast strains of Curvibasidium sp. isolated from medicinal lichen.
TL;DR: In this paper , two cold-adaptive strains of Curvibasidium sp., namely, Y230 and Y231, isolated from the medicinal lichen Usnea diffracta were investigated for their potential in lipid production.
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References
Lipid fermentation of corncob residues hydrolysate by oleaginous yeast Trichosporon cutaneum
TL;DR: This is the first report on using CCR as the feedstock for lipid fermentation and the lipid titer of 12.3g/L and dry cell weight of 38.4 g/L were the highest values among the studies using lignocellulose for lipid production.
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Efficiency of Conditionally Attenuated Salmonella enterica Serovar Typhimurium in Bacterium-Mediated Tumor Therapy
Michael Frahm,Sebastian Felgner,Dino Kocijancic,Manfred Rohde,Michael Hensel,Roy Curtiss,Marc Erhardt,Siegfried Weiss +7 more
TL;DR: The present study establishes a basis for the development of an applicably cancer therapeutic bacterium conditionally modified in their LPS phenotype that could represent a suitable vehicle to shuttle therapeutic compounds directly into cancerous tissue without harming the host.
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Microbial sources of polyunsaturated fatty acids (PUFAs) and the prospect of organic residues and wastes as growth media for PUFA-producing microorganisms
Maria Kothri,Maria Mavrommati,Ahmed M. Elazzazy,Mohamed N. Baeshen,Tarek A. A. Moussa,George Aggelis +5 more
TL;DR: It is concluded that several organic residues can be utilized as feedstock in the SCO production increasing the competitiveness of oleaginous organisms against conventional PUFA producers.
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Defective in Cuticular Ridges (DCR) of Arabidopsis thaliana, a Gene Associated with Surface Cutin Formation, Encodes a Soluble Diacylglycerol Acyltransferase
TL;DR: Data suggest that a broad specific DAG acyltransferase may be involved in the cutin as well as in the TAG biosynthesis by supplying hydroxy fatty acid.
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Microbial oil production from corncob acid hydrolysate by oleaginous yeast Trichosporon coremiiforme
TL;DR: Corncob acid hydrolysate was used for microbial oil production by oleaginous yeast Trichosporon coremiiforme for the first time and this strain is a promising strain for microbialOil production from lignocellulosic biomass.
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