Light-powered Escherichia coli cell division for chemical production.
Qiang Ding,Danlei Ma,Gao-Qiang Liu,Yang Li,Liang Guo,Cong Gao,Guipeng Hu,Chao Ye,Jia Liu,Liming Liu,Xiulai Chen +10 more
TL;DR: An optogenetic method is employed to realize dynamic morphological engineering of E. coli replication and division and shows the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).
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Abstract: Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm−1 and acetoin titer to 67.2 g·L−1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L−1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories. Manipulation of genes controlling microbial shapes can affect bio-production. Here, the authors employ an optogenetic method to realize dynamic morphological engineering of E. coli replication and division and show the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).
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Citations
Light-driven CO2 sequestration in Escherichia coli to achieve theoretical yield of chemicals
Guipeng Hu,Zehong Li,Danlei Ma,Chao Ye,Linpei Zhang,Cong Gao,Liming Liu,Xiulai Chen +7 more
- 29 Apr 2021
Abstract: CO2 sequestration engineering is an attractive strategy for achieving carbon- and energy-efficient bioproduction. However, the efficiency of heterotrophic CO2 sequestration is limited by bioproduct dependence and energy deficiency. Here, modular CO2 sequestration engineering was developed to produce target chemicals by integrating synthetic CO2 fixation and CO2 mitigation modules. A synthetic CO2 fixation pathway was designed, and then enhanced by light-driven reducing power using self-assembled cadmium sulfide nanoparticles. Next, a CO2 mitigation switch was designed, and then optimized by light-driven energy via proteorhodopsin. Finally, by integrating CO2 fixation and CO2 mitigation modules, the efficiency of CO2 sequestration was notably enhanced in Escherichia coli and the yields of l-malate and butyrate were increased to 1.48 and 0.79 mol/mol glucose, respectively, reaching theoretical yields. This CO2 sequestration system provides an efficient platform for channelling CO2 into value-added chemicals. Improving the efficiency of carbon yield in heterotrophic microorganisms is desired for biomanufacturing. Now, a product-independent and energy-efficient CO2 sequestration system that maximizes carbon conversion has been developed, as showcased by the production of chemicals reaching their theoretical yields.
115
Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli.
Xuan Wang,Jia-Ning Han,Xu Zhang,Yueyuan Ma,Yina Lin,Huan Wang,Dian-Jie Li,Tao-Ran Zheng,Fuqing Wu,Jianwen Ye,Jianwen Ye,Guo-Qiang Chen +11 more
TL;DR: In this article, a robust thermosensitive bio-switch with a post log-phase response and reversibility during scale-up bioprocesses is presented, which enables stringent bidirectional control of gene expression over time and levels in living cells.
The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments.
Sylvain Pouzet,Sylvain Pouzet,Sylvain Pouzet,Alvaro Banderas,Alvaro Banderas,Alvaro Banderas,Matthias Le Bec,Matthias Le Bec,Matthias Le Bec,Thomas Lautier,Thomas Lautier,Gilles Truan,Pascal Hersen,Pascal Hersen,Pascal Hersen +14 more
TL;DR: How optogenetics is currently applied to control metabolism in the context of bioproduction is discussed, the optogenetic instruments and devices used at the laboratory scale for strain development are described, and how current industrial-scale biopProduction processes could be adapted for optogenetically or could benefit from existing photobioreactor designs are explored.
55
Engineering AraC to make it responsive to light instead of arabinose.
Edoardo Romano,Edoardo Romano,Armin Baumschlager,Emir Bora Akmeriç,Navaneethan Palanisamy,Navaneethan Palanisamy,Moustafa Houmani,Gregor W. Schmidt,Mehmet Ali Öztürk,Leonard Ernst,Mustafa Khammash,Barbara Di Ventura +11 more
TL;DR: In this paper, an entire family of blue light-inducible AraC dimers in Escherichia coli (BLADE) are used to control gene expression in space and time.
52
Lignocellulosic biomass to biobutanol: Toxic effects and response mechanism of the combined stress of lignin-derived phenolic acids and phenolic aldehydes to Clostridium acetobutylicum
TL;DR: The proposed response mechanism of Com-Phe on solventogenic clostridia metabolism provides valuable guidance for developing robust strains and promotes cleaner biobutanol production from lignocellulosic biomass.
45
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