Journal Article10.1021/ACS.LANGMUIR.7B01020
Light-Controlled Membrane Mechanics and Shape Transitions of Photoswitchable Lipid Vesicles
Carla Pernpeintner,Carla Pernpeintner,James A. Frank,Patrick Urban,Christian R. Roeske,Stefanie D. Pritzl,Dirk Trauner,Dirk Trauner,Theobald Lohmüller,Theobald Lohmüller +9 more
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TL;DR: A protocol to form cell-sized vesicles from an azobenzene-containing phosphatidylcholine (azo-PC), which undergoes photoisomerization on irradiation with UV-A and visible light and a mechanism to utilize photoswitchable lipid membranes for storing mechanical energy and then releasing it on command as locally usable work is devised.
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Abstract: Giant unilamellar vesicles (GUVs) represent a versatile model system to emulate the fundamental properties and functions associated with the plasma membrane of living cells. Deformability and shape transitions of lipid vesicles are closely linked to the mechanical properties of the bilayer membrane itself and are typically difficult to control under physiological conditions. Here, we developed a protocol to form cell-sized vesicles from an azobenzene-containing phosphatidylcholine (azo-PC), which undergoes photoisomerization on irradiation with UV-A and visible light. Photoswitching within the photolipid vesicles enabled rapid and precise control of the mechanical properties of the membrane. By varying the intensity and dynamics of the optical stimulus, controlled vesicle shape changes such as budding transitions, invagination, pearling, or the formation of membrane tubes were achieved. With this system, we could mimic the morphology changes normally seen in cells, in the absence of any molecular machines...
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Citations
Investigating the Influence of Photoswitchable Lipids on the Structure and Dynamics of Lipid Membranes: Fundamentals and Potential Applications.
TL;DR: The feasibility of photoactivated drug release, exemplified by the controlled liberation of doxorubicin through the membrane, is demonstrated, suggesting the potential of PSLs in engineering photoactivated liposomes, coined as photoazosomes, for precise targeted drug delivery applications.
Photoliposomes; Suitable Platforms for Studying the Order and Dynamics of Lipid Bilayers and Integral Proteins by MAS-NMR Spectroscopy
Mahmoudreza Doroudgar
- 02 May 2022
TL;DR: In this paper , a specific azobenzene containing photolipid (AzoPC) is integrated into POPE:POPG bilayers, and it is shown that DgkA enhances the AzoPC localization impact on the fluidity of the bulk lipids.
Synthetic studies of archaeal lipids and related long-chain isoprenoids
Ruben L. H. Andringa
- 02 Jun 2022
TL;DR: In this article , the authors employed the art and science of total synthesis as a framework to gain a better understanding of the biosynthesis of archaeal lipids, and provided synthetic molecules that they in turn can use to investigate biosynthetic processes.
Endocytosis-like vesicle fission by membrane-expanding molecular machine enabling virus encapsulation for in vivo delivery
30 Sep 2022
TL;DR: In this article , a membrane-expanding molecular machine containing a diazocine core (AzoMEx) exhibits an opening/closing mechanical motion in response to visible light.
Phase behaviour and adsorption of deoxyribonucleic acid onto an azobenzene liquid crystalline ligand at the interfaces
TL;DR: In this paper , the phase behavior of the C7 ALC ligand at the air-water (A-W) interface is investigated employing surface manometry, and the surface pressure-area per molecule isotherm shows that the phase sequence is liquid expanded, liquid condensed, and then collapse to three-dimensional crystallites.
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