Large needle aspiration biopsy and galectin-3 determination in selected thyroid nodules with indeterminate FNA-cytology
Angelo Carpi,Antonio Giuseppe Naccarato,Giorgio Iervasi,Andrea Nicolini,Generoso Bevilacqua,Paolo Viacava,Paola Collecchi,Luca Lavra,Clorinda Marchetti,Salvatore Sciacchitano,Armando Bartolazzi +10 more
TL;DR: LNAB plus galectin-3 expression analysis when applied preoperatively to selected thyroid nodules candidate to surgery can potentially reduce unnecessary thyroid resections.
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Abstract: Thyroid fine-needle aspiration biopsy (FNA)-cytology is widely used for the preoperative characterisation of thyroid nodules but this task is difficult for follicular lesions, which often remain undefined. We propose a strategy for improving the preoperative characterisation of selected follicular thyroid proliferations, which is based on large needle aspiration biopsy (LNAB) and galectin-3 expression analysis. Eighty-five thyroid specimens were obtained by LNAB (20-gauge needles) from thyroid nodules with indeterminate follicular FNA-cytology. Aspirated material was processed as a tissue microbiopsy to obtain cell blocks for both cyto/histo-morphological evaluation and galectin-3 expression analysis, by using a purified monoclonal antibody to galectin-3 and a biotin-free immunoperoxidase staining method. Preoperative diagnosis was compared to the final histology. LNAB and cell-block technique allow a preliminary distinction between nodules with a homogeneous microfollicular/trabecular structure, as frequently observed in tumours, and lesions with mixed normo–micro–macrofollicular architecture, as observed in goitre. Furthermore, LNAB provides optimal substrates for galectin-3 expression analysis. Among 85 cases tested, 14 galectin-3-positive cases were discovered preoperatively (11 thyroid cancers and three adenomas confirmed at the final histology), whereas galectin-3-negative cases were 71 (one carcinoma and 70 benign proliferations at the final histology). Sensitivity, specificity and diagnostic accuracy of this integrated morphologic and phenotypic diagnostic approach were 91.6, 97.2 and 95.3%, respectively. In conclusion, LNAB plus galectin-3 expression analysis when applied preoperatively to selected thyroid nodules candidate to surgery can potentially reduce unnecessary thyroid resections.
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Citations
Galectin-3: One Molecule for an Alphabet of Diseases, from A to Z.
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TL;DR: The current review aims to extensively examine the studies published so far on the role of Gal-3 in all the clinical conditions and diseases, listed in alphabetical order, where it was analyzed.
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References
Controversies in diagnostic approaches to the indeterminate follicular thyroid nodule.
Angelo Carpi,A. Nicolini,Milton D. Gross,Lorraine M. Fig,Brahm Shapiro,Stefano Fanti,Lucia Rampin,Cesare Polico,D. Rubello +8 more
TL;DR: In this article, the authors review the controversies in diagnostic approaches to follicular thyroid nodules with indeterminate cytology, including the definition and the postoperative outcome, and suggest some techniques that may improve preoperative diagnostic accuracy are large needle aspiration biopsy and immunohistochemistry for galectin-3.
22
Diagnostic Applicability of Dipeptidyl Aminopeptidase IV Activity in Cytological Samples for Differentiating Follicular Thyroid Carcinoma From Follicular Adenoma
TL;DR: Positive DPP IV activity in cytological samples is the best discriminatory marker between follicular thyroid carcinoma and follicular adenoma and its application could alter the clinical management of patients with follicle thyroid tumors.
17
•Journal Article
Baja capacidad de galectina-3 para discriminar lesiones tiroideas
Romulo De-León-Mazariegos,Marcela Canedo-Patzi,Bernardo Pérez-Enríquez,Fernando Candanedo-Gonzalez,Milena Saqui-Salces,Armando Gamboa-Domínguez,Juan Antonio Rull-Rodrigo +6 more
TL;DR: Galectina-3 (NCL-GAL3, 1: 100, Novocastra, UK) as discussed by the authors was used to identify lesiones tiroideas benignas de malignas.
3
•Journal Article
Increasing the accuracy of fine-needle biopsy for thyroid nodules.
TL;DR: A criterion was established for adequacy of an FNB specimen (ie, at least six clusters of benign cells on each of two slides prepared from separate aspirations) in an attempt to reduce the rate of false-negative diagnoses attributable to inadequate sampling.