Journal Article10.1039/C5CC05410A
Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity.
Qingwang Xue,Lei Wang,Wei Jiang +2 more
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TL;DR: A one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase activity detection is developed.
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About: This article is published in Chemical Communications. The article was published on 18 Aug 2015. The article focuses on the topics: Molecular beacon.
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Citations
Sensitive fluorescent detection of DNA methyltransferase using nicking endonuclease-mediated multiple primers-like rolling circle amplification.
TL;DR: Improved RCA amplification efficiency and specific fluorescent recognition of RCA products provide the M.SssI MTase-sensing platform with high sensitivity and is applicable for screening and evaluating the inhibitory activity of MTase inhibitors.
86
Collapse of DNA Tetrahedron Nanostructure for “Off–On” Fluorescence Detection of DNA Methyltransferase Activity
Xiaoyan Zhou,Xiaoyan Zhou,Min Zhao,Xiaolei Duan,Bin Guo,Wei Cheng,Shijia Ding,Huangxian Ju,Huangxian Ju +8 more
TL;DR: A novel multifunctional nanostructure has been developed as an "off-on" fluorescent probe for detection of target methyltransferase by integrating the elements of DNA tetrahedron, target recognition, and dual-labeled reporter in the field of clinical diagnostics.
57
Dual-Amplification Strategy-Based SERS Chip for Sensitive and Reproducible Detection of DNA Methyltransferase Activity in Human Serum.
TL;DR: A dual-amplification sensing strategy-based surface-enhanced Raman scattering (SERS) chip, which combines rolling circle amplification (RCA) and polyadenine (PolyA) assembly for sensitive and reproducible determination of the activity of M.SssI with various concentrations spiked in human serum samples with good recoveries.
49
Highly effective molecule converting strategy based on enzyme-free dual recycling amplification for ultrasensitive electrochemical detection of ATP
TL;DR: An enzyme-free and highly effective molecule converting strategy based on target-driven catalytic hairpin assembly and Mg2+-dependent DNAzyme recycling dual-amplification was proposed to construct an ultrasensitive electrochemical biosensor for adenosine triphosphate (ATP) detection.
48
Highly Sensitive and Quality Self-Testable Electrochemiluminescence Assay of DNA Methyltransferase Activity Using Multifunctional Sandwich-Assembled Carbon Nitride Nanosheets
TL;DR: A sandwich-assembled electrochemiluminescence (ECL) biosensor using multifunctional carbon nitride nanosheets (CNNS) to evaluate the MTase activity and highlighted the great potential of emerging CNNS luminophor in developing highly sensitive and smart quality self-testable ECL sensing systems using a sandwiched configuration for early disease diagnosis, treatment, and management.
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