Book Chapter10.1016/S0074-7696(08)61426-6
Kinetoplast DNA in trypanosomid flagellates.
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TL;DR: This chapter discusses the kinetoplast DNA in trypanosomid flagellates and focuses on the general characteristics of minicircle DNA of several species, including Trypanosoma brucei, TrypanOSoma equiperdum, Leishmania tarentolae,Crithidia fasciculata, and Crithidia luciliae.
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Abstract: Publisher Summary This chapter discusses the kinetoplast DNA in trypanosomid flagellates. The chapter reviews the recent developments in the application of recombinant DNA technology to problems of the structure, replication, and transcription of the unusual mitochondrial DNA in the kinetoplastid protozoa known as “kinetoplast DNA.” Kinetoplast DNA is discussed in terms of its two molecular components—minicircles and maxicircles, with an emphasis on species-dependent variations. The chapter focuses on the general characteristics of minicircle DNA of several species, including Trypanosoma brucei, Trypanosoma equiperdum, Leishmania tarentolae, Crithidia fasciculata, and Crithidia luciliae. Unlike most other kinetoplastid species, the kDNA minicircles from T.equiperdum are homogeneous in base sequence. The minicircles in the Leishmania are the smallest reported to date. L .tarentolae minicircles are about 870 bp in size and show extensive sequence heterogeneity by restriction enzyme analysis. The minicircles in the Leishmania are the smallest reported to date. L.tarentolae minicircles are about 870 bp in size and show extensive sequence heterogeneity by restriction enzyme analysis. The maxicircle component of the kinetoplast DNA appears to represent the homolog of the informational mitochondria1 DNA found in other eukaryotic cells.
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Citations
The Mechanism of U Insertion/Deletion RNA Editing in Kinetoplastid Mitochondria
TL;DR: Circumstantial in vitro evidence for involvement of an RNA ligase and an endoribonuclease, which are components of a 20S complex, was obtained.
The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis.
Marcello Ceccarelli,Luca Galluzzi,Aurora Diotallevi,Francesca Andreoni,Hailie Fowler,Christine A. Petersen,Fabrizio Vitale,Mauro Magnani +7 more
TL;DR: A new and affordable SYBR-green qPCR-based approach to distinguish between L. (L.) infantum and L.(L.) amazonensis was developed exploiting the major abundance of a minicircle sequence rather than targeting a hypothetical species-specific sequence.
Analysis of sequences of two different classes of Kinetoplast DNA minicircles of a Leishmania Spp.
TL;DR: The heterogeneity of minicircle sequences in the variable regions may be exploited in developing recombinant DNA based diagnostic probes for detection and classification of Leishmania species.
PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA
Feng-Jun Li,Robin B. Gasser,Robin B. Gasser,De-Hua Lai,Filip Claes,Xing-Quan Zhu,Zhao-Rong Lun +6 more
TL;DR: A PCR approach based on the sequence of maxicircle kinetoplast DNA of Trypanosoma brucei to distinguish T. Brucei/T.
Isolation of proteins associated with kinetoplast DNA networks in vivo.
Chanxing Xu,Dan S. Ray +1 more
TL;DR: It is suggested that basic proteins may play a role in the condensation of kDNA in the kinetoplast and that these proteins are imported into the kinEToplast by a mechanism involving a cleavable presequence.
References
The locus of sequence-directed and protein-induced DNA bending
Hen-Ming Wu,Donald M. Crothers +1 more
TL;DR: The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence symmetrically distributed about it, with a repeat interval of 10 base pairs.
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Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids.
TL;DR: Etude des differents elements helicoidaux : Structures d'ordre superieur a la structure secondaire.
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André Lwoff,S. H. Hutner +1 more
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TL;DR: Biochemistry and physiology of protozoa is studied in detail in order to clarify the role of “cell reprograming” in the “building blocks” of disease.
623
Origins of prokaryotes, eukaryotes, mitochondria, and chloroplasts
TL;DR: The symbiotic theory of cellular evolution is considered the most probable because it holds that organelles, e.g., mitochondria and chloroplasts invaded larger bodies, and combined functions to form eucaryotic cells.
573
Strains and clones of Trypanosoma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles.
TL;DR: It is shown that, from a given T. cruzi strain, clones with different biological properties can be isolated and identified by their restriction patterns.
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