Patent
Isoprene synthase variants for improved microbial production of isoprene
Richard R. Bott,Marguerite A. Cervin,James T. Kellis,Joseph C. Mcauliffe,Andrei Miasnikov,Caroline M. Peres,Christopher L. Rife,Derek H. Wells,Walter Weyler,Gregory M. Whited +9 more
- 23 Apr 2009
89
TL;DR: In this article, the authors present methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility for increased isopene production in microbial host cells.
read more
Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Microbial production of isoprenoids
TL;DR: This review will deconstruct the processes by which microbial production of certain isoprenoids was achieved, and represent a wide range of functionalities and applications, and also allow the different routes taken for their successful biosyntheses.
131
Patent
Compositions and methods for producing isoprene
Marguerite A. Cervin,Gopal K. Chotani,Frank James Feher,Richard La Duca,Joseph C. McAuliffe,Andrei Miasnikov,Caroline M. Peres,Aaron Scott Puhala,Karl J. Sanford,Fernando Valle,Gregory M. Whited +10 more
- 15 Dec 2008
TL;DR: In this paper, a method for producing isoprene from cultured cells is described, and compositions that include these cultured cells are also provided, including compositions that can be used to synthesize isoprenes.
101
Engineering microbes for isoprene production.
Lidan Ye,Xiaomei Lv,Hongwei Yu +2 more
TL;DR: The history and prospects of microbial isoprene production is summarized by summarizing the history, prospects, and strategies employed for achieving engineered strains with improved performance indices based on the published papers and patents.
88
Patent
Microorganisms and methods for the biosynthesis of butadiene
Mark J. Burk,Anthony P. Burgard,Jun Sun,Robin E. Osterhout,Priti Pharkya +4 more
- 04 May 2011
TL;DR: In this article, the authors provided non-naturally occurring microbial organisms having a butadiene pathway and provided methods of using such organisms to produce butadiENE, and they also provided methods for using these organisms to generate butadienes.
62
Patent
Fuel compositions comprising isoprene derivatives
Joseph C. Mcauliffe,Sergey Paramonov,Karl J. Sanford +2 more
- 17 Jun 2010
TL;DR: In this article, the authors describe methods, compositions and systems using bioisoprene derived from renewable carbon for production of a variety of hydrocarbon fuels and fuel additives. But they do not discuss the use of bio-diesel.
58
References
The 1-deoxy-d-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants
Hartmut K. Lichtenthaler
- 01 Jun 1999
TL;DR: The DOXP pathway of IPP formation starts from D-glyceraldehyde-3-phosphate and pyruvate, with DOXP-synthase as the starting enzyme, and provides new insight into the regulation of chloroplast metabolism.
1.4K
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.
TL;DR: An improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins, successfully applied for theinternal sequence analysis of membrane proteins from the rat mitochondria resolved in preparative two-dimensional gel electrophoresis.
1.3K
The worldwide Protein Data Bank (wwPDB): ensuring a single, uniform archive of PDB data
TL;DR: The worldwide Protein Data Bank (wwPDB) is the international collaboration that manages the deposition, processing and distribution of the PDB archive, a repository for the coordinates and related information for more than 38 000 structures, including proteins, nucleic acids and large macromolecular complexes that have been determined using X-ray crystallography, NMR and electron microscopy techniques.
Stability effects of mutations and protein evolvability
Nobuhiko Tokuriki,Dan S. Tawfik +1 more
TL;DR: Way of predicting and analyzing stability effects of mutations, and mechanisms that buffer or compensate for these destabilizing effects and thereby promote protein evolvabilty, in nature and in the laboratory are described.
1K