Journal Article10.1089/TEN.TEA.2009.0529
Isolation and characterization of multipotent rat tendon-derived stem cells.
TL;DR: The successful isolation of tendon-derived stem cells under the optimized growth and differentiation conditions was useful for future stem-cell-based tissue regenerative studies as well as studies on their roles in tendon physiology, healing, and disorders using the rat model.
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Abstract: Stem cells have recently been isolated from humans and mice but not from rat tendon tissue. This study reports the isolation and characterization of stem cells from rat tendon. Nucleated cells isolated from rat flexor tendon tissues after collagenase digestion were plated at a low cell density to allow the selective proliferation of tendon-derived stem cells. About 1-2% of the cells isolated under this optimized culturing condition showed clonogenicity, high proliferative potential at low seeding density, and osteogenic, chondrogenic, and adipogenic multidifferentiation potential. These cells were CD44(+), CD90(+), CD34(-), and CD31(-). Although they shared some common properties with mesenchymal stem cells, they also exhibited their unique characteristics by expressing tenogenic and chondrogenic markers. There was expression of tenogenic markers, including alpha-smooth muscle actin, tenascin C, and tenomodulin, but not collagen type I at passage 0 (P0) and P3. Expression of a chondrogenic marker, aggrecan, was observed at P0 and P3, whereas expression of collagen type II was observed in few cells only at P3. The successful isolation of tendon-derived stem cells under the optimized growth and differentiation conditions was useful for future stem-cell-based tissue regenerative studies as well as studies on their roles in tendon physiology, healing, and disorders using the rat model.
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Citations
Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase.
TL;DR: SF films with a bionic microstructure may serve as a scaffold, provide biophysical cues to alter the cellular adherence arrangement and cell morphology, and enhance the tenogenic gene and protein expression in TSPCs.
Differentiation Ability of Tendon-Derived Stem Cells and Histological Characteristics of Rotator Cuff Remnant on the Greater Tuberosity Degenerated With Age and Chronicity
Janne Poikolainen,Jaclyn A. Kerr +1 more
- 01 Apr 2022
TL;DR: In this article , the presence and activity of tendon-derived stem cells (TDSCs), as well as histological changes of rotator cuff remnant by age and chronicity of the RCT were explored and measured.
Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells.
Fan Lai,Wang Jingjing,Hong Tang,Bian Xuting,Kang Lu,Gang He,Pan Huang,Juan Liu,Mei Zhou,Jian Liu,Xu Tao,Kanglai Tang +11 more
TL;DR: It is not likely that the adipocyte accumulation in aging tendon during repair was due to the aging of TSPCs, and this may provide new targets for curing aging tendon injuries or tendinopathies.
Biology and mechano‐response of tendon cells: Progress overview and perspectives
TL;DR: The focus of the discussions ranged from current research progress, challenges and opportunities, to future directions on these topics, which included the biology of tendon stem/progenitor cells and the potential of stem cell‐based tendon therapy using TSPCs and other types of stem cells.
Various Strategies of Tendon Stem/Progenitor Cell Reprogramming for Tendon Regeneration
Sung Yong Ahn
TL;DR: This review discusses novel markers and strategies for reprogramming tendon stem/progenitor cells (TSPCs) to regenerate rotator cuff tendons, a common cause of shoulder pain, and potentially treat age-related degenerative diseases through regenerative medicine.
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Human Adipose Tissue Is a Source of Multipotent Stem Cells
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