Isolation and characterization of a mannan-binding protein from rabbit liver

TL;DR: In this article, a composite of mannan binding protein attached to an insoluble, support matrix is obtained by reacting cyanogen bromide activated beaded agarose with a buffered solution of protein isolated from rabbit serum, which has utility as an affinity sorbent for IgM when divalent metal ions are incorporated in the binding buffer.

TL;DR: An enzyme-linked immunosorbent assay was developed and data strongly suggested that hepatocyte may be one of the production sites of this glycoprotein, which was detected by an autoantibody in sera of a Japanese woman with systemic lupus erythematosus.

TL;DR: It is reported that equine MBL (eMBL) has properties that are similar to human MBL, and low levels of MBL:MASP activity in sick horses compared to healthy horses are found, suggesting that eMBL is involved in the immune response of the horse and that low MBL-associated serine protease-2 activity could be used to monitor immune function and clinical outcome.

TL;DR: The cyclic clusters proved to be superactive and in a manner suggestive of a cooperative response of the membrane receptors to release lysozyme from human neutrophils.

TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.

TL;DR: Inherent advantages of this method of purification are the rapidity and ease of a potentially single-step procedure, the rapid separation of the protein to be purified from inhibitors and destructive contaminants, such as proteases, and protection from denaturation during purification by active site ligand-stabilization of protein tertiary structure.

TL;DR: Of all the biologically occurring macromolecules the glycoproteins, which consist of carbohydrate moieties convalently linked to a polypeptide backbone, represent the most diverse group, ranging from substances in which the carbohydrate component represents less than 1% of the total weight to those in which it represents over 80% ofThe total.

TL;DR: The purification, by affinity chromatography, of an hepatic protein which retains the characteristic binding properties associated with the membranes is described, which indicates a high degree of aggregation in the final, water-soluble preparation.