Isolation and characterization of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide Vmw110
Nigel D. Stow,Elizabeth C. Stow +1 more
454
TL;DR: The results suggest that the effect of the Vmw110 deletion is manifest primarily at low multiplicities of infection and can be largely overcome by increasing the virus dose.
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Abstract: Transfection experiments with plasmids containing immediate early (IE) genes of herpes simplex virus type 1 (HSV-1) have previously demonstrated a role for the IE polypeptide Vmw110 (ICP0) in stimulating expression from plasmid-encoded early gene promoters. To gain further insights into the function of Vmw110 we isolated a deletion mutant specifying a truncated form of the polypeptide which had been shown to be inactive in transfection assays. This mutant, dl1403, contained a 2 kb deletion within both the TRL and IRL copies of the Vmw110 gene, and encoded a polypeptide consisting of the original N-terminal 105 amino acids followed by 56 amino acids specified by a reading frame not used by Vmw110. dl1403 was able to replicate and produce plaques on baby hamster kidney (BHK) cells but the yield of infectious virus was 20- to 100-fold lower than obtained with wild-type HSV-1. Surprisingly, comparison of polypeptide synthesis, DNA replication and DNA encapsidation in cells infected with 5 p.f.u./cell dl1403 or wild-type HSV-1 revealed no significant differences. In addition similar numbers of particles were produced in cells infected with the two viruses, resulting in stocks of dl1403 exhibiting significantly higher particle/p.f.u. ratios. The efficiency of plaquing of dl1403 was greatly reduced in Vero and human foetal lung cells compared with BHK cells, but following infection with 5 p.f.u./cell similar yields of infectious virus were obtained from all three cell lines. Marker rescue experiments verified that the reduced yield of dl1403 in BHK cells was a consequence of the deletion within the Vmw110 gene. The results suggest that the effect of this deletion is manifest primarily at low multiplicities of infection and can be largely overcome by increasing the virus dose.
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A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory protein
TL;DR: At early times of virus infection, the presence of Vmw110 increases the proportion of ND10 which contain the ubiquitin‐specific protease, and these results identify a novel, transitory component ofND10 and implicate a previously uncharacterized ubiquitIn‐dependent pathway in the control of viral gene expression.
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The Disruption of ND10 during Herpes Simplex Virus Infection Correlates with the Vmw110- and Proteasome-Dependent Loss of Several PML Isoforms
Roger D. Everett,Paul S. Freemont,Hisato Saitoh,Mary Dasso,Anne Orr,Meeta Kathoria,Jane Parkinson +6 more
TL;DR: The results show that the disruption of ND10 during virus infection correlates with the loss of several PML isoforms and this process is dependent on active proteasomes, suggesting a simple yet powerful mechanism by which Vmw110 might function duringirus infection.
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References
Generation of an inverting herpes simplex virus 1 mutant lacking the L-S junction a sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the alpha 47 gene.
TL;DR: It is concluded that the four unique genes deleted in R7023 are not essential for the growth of herpes simplex virus, at least in the cell lines tested, and that the b sequence of the inverted repeats of the L component also contains cis-acting sites for the inversion of herpessimplex virus DNA sequences.
176
Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides.
TL;DR: Evidence is presented that the immediate early polypeptide Vmw IE 110 of HSV-1 and its functionally equivalent polypeptic may map in the repetitive sequences bounding the long unique region ofHSV.
167
The terminal a sequence of the herpes simplex virus genome contains the promoter of a gene located in the repeat sequences of the L component.
J Chou,Bernard Roizman +1 more
TL;DR: It is reported that the 500-base-pair a sequence contains the promoter-regulatory domain and the transcription initiation site of a diploid gene, the coding sequences of which are located in the b sequences of the inverted repeats of the L component.
159
The synthesis and substructure of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA.
TL;DR: The results show that the ‘intact’ strands reassociated with the same kinetics and to the same extent as total virus DNA, suggesting that both strands of the duplex were present in equal amounts.
137
DNA sequence analysis of an immediate-early gene region of the herpes simplex virus type 1 genome (map coordinates 0.950 to 0.978).
M.-J. Murchie,D. J. Mcgeoch +1 more
TL;DR: The nucleotide sequence of 4 kilobases of DNA from within the short region of the genome of herpes simplex virus type 1 has been determined and shown to be flanked by sequences which resemble those found in similar positions in other eukaryotic genes.
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