Journal Article10.1016/J.CHROMA.2007.09.047
Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds?
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TL;DR: The separation of acidic, neutral and particularly basic solutes was investigated using a bare silica column, mostly under hydrophilic interaction chromatography (HILIC) conditions with water concentrations >2.5% and with >70% acetonitrile (ACN).
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About: This article is published in Journal of Chromatography A. The article was published on 09 Nov 2007. The article focuses on the topics: Hydrophilic interaction chromatography & High-performance liquid chromatography.
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Citations
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more
TL;DR: Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases and their applications for separations of polar compounds in complex matrices.
Stationary and mobile phases in hydrophilic interaction chromatography: a review
TL;DR: This review deals with recent advances in the development of HILIC phase separation systems with special attention to the properties of stationary phases.
683
Current approaches and challenges for the metabolite profiling of complex natural extracts
TL;DR: A survey of the techniques that are used for generic and comprehensive profiling of secondary metabolites in natural extracts is provided and the structural information that can be generated through these techniques or in combination is compared in relation to the identification of metabolites in complex mixtures.
510
Retention and selectivity of stationary phases for hydrophilic interaction chromatography.
Yong Guo,Sheetal Gaiki +1 more
TL;DR: This review provides a survey of the popular stationary phases commercially available and discusses the retention and selectivity characteristics of the polar stationary phases in HILIC.
315
Study of the selectivity, retention mechanisms and performance of alternative silica-based stationary phases for separation of ionised solutes in hydrophilic interaction chromatography.
TL;DR: Differences in selectivity between these various columns indicate that the stationary phase cannot function merely as an inert support for a water layer into which the solutes partition from the bulk mobile phase.
247
References
Hydrophilic interaction chromatography using amino and silica columns for the determination of polar pharmaceuticals and impurities.
TL;DR: An examination of the retention and separation of several pyrimidines, purines, and amides on silica and amino columns from three manufacturers revealed that mobile phases should contain a buffer or acid for pH control to achieve similar and reproducible results among columns from different sources.
Practice and mechanism of HPLC oligosaccharide separation with a cyclodextrin bonded phase
TL;DR: It is shown that partitioning interactions and hydrogen bonding interactions between the hydroxyl groups of the stationary phase and those of the sugars are the two possible mechanisms responsible for sugar retention.
Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases.
TL;DR: This review article summarizes the recent progress on bioanalytical LC-MS/MS methods using underivatized silica columns and aqueous/organic mobile phases and proves the silica column demonstrated superior column stability.
•Book
The Essence of Chromatography
Colin F. Poole
- 19 Dec 2002
TL;DR: General concepts in column chromatography The column in gas chromatography and column in liquid chromatography Instrumental aspects of liquid chromalysis Thin-layer chromatography Supercritical fluid chromatography Spectroscopic detectors for identification and quantification Separation of stereoisomers Laboratory-scale preparative chromatography as mentioned in this paper.