Journal Article10.1002/JBM.A.34039
Interstitial interfaces show marked differences in regenerating tubules, matured tubules, and the renal stem/progenitor cell niche.
Will W. Minuth,Lucia Denk +1 more
TL;DR: In this article, the interstitial interface of the renal stem/progenitor cells was analyzed by electron microscopy in comparison with the interstitium of matured tubules.
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Abstract: Stem/progenitor cells are promising candidates for the regeneration of parenchyma in acute and chronic renal failure. After an implantation stem/progenitor cells must migrate through the interstitial space to concentrate at the site of damage. However, information is lacking to what extent the interstitial interface is influencing the development of stem/progenitor cells into nephron structures. In consequence, tubule regeneration within an artificial polyester interstitium was analyzed by electron microscopy in comparison with the interstitial interface of matured tubules and the interstitium within the renal stem/progenitor cell niche. The experiments demonstrate that fixation of specimens with glutaraldehyde (GA) is leading in all cases to inconspicuously looking interstitial interfaces. In contrast, fixation of regenerating tubules in GA containing ruthenium red and tannic acid shows a dense network of fibers lining along the basal lamina. In contrast, matured tubules reveal after ruthenium red label an extremely thickened basal lamina, while only a punctate pattern is obtained after tannic acid treatment. Finally, within the renal stem/progenitor cell niche ruthenium red and tannic acid label reveals large amounts of extracellular matrix spanning through the interstitium. Thus, fixation of tissue in GA containing ruthenium red and tannic acid exhibits an unexpectedly regional heterogeneity of the renal interstitial interface. This fact has to be considered for an optimal therapeutic repair of parenchyma, since contacts between stem/progenitor cells with the interstitial interface influence further development.
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Citations
Supportive development of functional tissues for biomedical research using the MINUSHEET® perfusion system
Will W. Minuth,Lucia Denk +1 more
TL;DR: The results point out that the cell biological profile of engineered tissues can be strongly improved, when the introduced perfusion culture technique is applied in combination with specific biomaterials supporting primary adhesion of cells.
Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system.
Will W. Minuth,Lucia Denk +1 more
TL;DR: To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed and applied in numerous own and external investigations.
Initial steps to stabilize the microenvironment for implantation of stem/progenitor cells in diseased renal parenchyma
Will W. Minuth,Lucia Denk +1 more
- 28 Sep 2013
TL;DR: Stem/progenitor cells are in the focus of research as a therapeutic perspective to cure acute and chronic renal failure from a stem cell perspective and following this innovative strategy one has to consider that stem/progener cells are first kept in the body.
Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules
Will W. Minuth,Lucia Denk +1 more
TL;DR: The actual data show that development of abnormal cell features must be taken into account, when regeneration of renal tubules is simulated under in vitro conditions.
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The rabbit nephrogenic zone in culture: past, present and future as a model to investigate causes of impaired nephrogenesis
Will W. Minuth
- 07 Dec 2016
TL;DR: In preterm infants, intrauterine as well as extrauterine influences are held responsible for causing prematurity of renal parenchyma and impaired nephrogenesis, leading to a high incidence of severe kidney diseases later in life, and isolation and culture of the nephrogensic zone from neonatal rabbit kidney is particularly well-suited.
5
References
Epithelial-mesenchymal transition (EMT) in kidney fibrosis: fact or fantasy?
TL;DR: It is the view that unequivocal evidence supporting EMT as an in vivo process in kidney fibrosis is lacking and both sides of this debate are reviewed.
Fibroblast activation and myofibroblast generation in obstructive nephropathy
TL;DR: The molecular mechanisms of fibroblast activation and epithelial–mesenchymal transition are examined, processes that seem to be promising targets for the prevention, or even reversal, of interstitial fibrosis and renal dysfunction associated with obstructive nephropathy.
275
Role of inflammation in túbulo-interstitial damage associated to obstructive nephropathy
María T. Grande,María T. Grande,Fernando Pérez-Barriocanal,Fernando Pérez-Barriocanal,José M. López-Novoa,José M. López-Novoa +5 more
TL;DR: The different events and regulatory mechanisms involved in inflammation associated to obstructive nephropathy are revised and increased angiotensin II production, increased oxidative stress and high levels of proinflammatory cytokines are revised.
166
The ultrastructural organization and properties of the mouse tectorial membrane matrix
Judith A Hasko,Guy P. Richardson +1 more
TL;DR: Tannic acid treatment reveals that the matrix both in between and within the Type A protofibril bundle system has a high degree of structural organization, indicating that the structure can undergo at least one cycle of depolymerization and polymerization in vitro.
121
Development of tubular and glomerular cells of the kidney
Lydia Sorokin,Peter Ekblom +1 more
TL;DR: The kidney is an ideal model for studying the establishment of polarity of epithelial cells, a developmentally important event also shared by cells which develop by branching morphogenesis.
94