Journal Article10.1080/09553000010028476
In vivo chromosomal instability and transmissible aberrations in the progeny of haemopoietic stem cells induced by high- and low-LET radiations
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TL;DR: Chromosomal instability induced in vitro can be transmitted in vivo by transplantation of haemopoietic stem cells exposed to high- or low-LET radiations and comparable instability can be induced and shown to persist for the remaining lifetime after whole-body irradiation.
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Abstract: Purpose : To study stable and unstable chromosomal aberrations in the haemopoietic cells of CBA/H mice after exposure to both high- and low-LET radiations. Materials and methods : Chromosomal aberrations were scored in the clonal progeny of X-, α - or non-irradiated short-term repopulating stem cells using the spleen colony-forming unit (CFU-S) assay, 12 days post-transplantation and in the bone marrow reconstituted by X-, neutron- or non-irradiated exogenous (transplanted) or endogenous (X- or neutron whole-body-irradiated) long-term repopulating stem cells for up to 24 months. Results : Chromosomal instability was demonstrated in 3-6% of cells in all cases. After transplantation of X- or neutron-irradiated bone marrow ~8% of cells with stable aberrations were recorded at all times. After 3 Gy X- or 0.5Gy neutron- whole-body irradiation stable aberrations were detected in ~17 and 5% of cells respectively. Conclusions : Chromosomal instability induced in vitro can be transmitted in vivo by transplantation...
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TL;DR: In this paper, retrovirus-mediated gene transfer was used to mark hematopoietic stem cells in vitro and have tracked the fate of these cells after their transplantation into lethally irradiated recipients.
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•Journal Article
Delayed chromosomal instability induced by DNA damage
TL;DR: In this paper, the authors used fluorescence in situ hybridization to detect chromosomal rearrangements in a human/hamster somatic hybrid cell line following exposure to ionizing radiation.
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Delayed chromosomal instability induced by DNA damage
B.A. Marder,William F. Morgan +1 more
TL;DR: The types of chromosomal rearrangements observed suggest that chromosome fusion, followed by bridge breakage and refusion, contributes to the observed delayed chromosomal instability, and a role for chromosome rearrangement in cytotoxicity is suggested.
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α-particle-induced chromosomal instability in human bone marrow cells
TL;DR: Non-clonal cytogenetic aberrations are found in clonal descendants of haemopoietic stem cells after exposure to alpha-particles of bone marrow cells from two of four haematologically normal individuals, consistent with a transmissible genetic instability induced in a stem cell resulting in a diversity of aberration in its clonal progeny many cell divisions later.
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Two Phases of Engraftment Established by Serial Bone Marrow Transplantation in Mice
TL;DR: It was shown that serial bone marrow transplantation changed the ratio of hematopoietic progenitors in bone marrow, and the numbers of granulocyte-macrophage colony-forming units (CFU-GM) in the bone marrow did not change with serial transplantation.
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