Journal Article10.1111/J.1749-6632.1995.TB19944.X
In Vitro Protein Biosynthesis Using Ribosome and Foreign mRNAa
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About: This article is published in Annals of the New York Academy of Sciences. The article was published on 01 Mar 1995. The article focuses on the topics: Initiation factor & Protein biosynthesis.
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Citations
Continuous-Exchange Protein-Synthesizing Systems
TL;DR: The basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described, and the ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed.
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Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors
V.A. Shirokov,Peter Nikolayevich Moscow Region Simonenko,Sergey Vladimirovich Biryukov,Alexander S. Spirin +3 more
- 01 Jan 2002
TL;DR: This work states that gene expression in vitro, in cell-free translation or transcription-translation systems, is an alternative that allows full control and high flexibility of conditions.
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TL;DR: Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography.
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