In vitro gene expression within membrane-free coacervate protocells

TL;DR: Evidence that coacervation could have played a unique role during the origin of life based on its ability to form from a range of different prebiotically relevant molecules and partition solutes and maintain the solute’s partition coefficient during enzymatic reactions is provided.

TL;DR: A bio-inspired inorganic synzyme is employed to construct a micro-compartment with multi-functional activity providing a step towards the development of protocell reaction networks.

TL;DR: It is shown that RNA signals transcribed from transcription organelles can be specifically targeted to capture organellees via hybridization to the corresponding DNA addresses, and it is demonstrated that mRNA molecules are only translated in translation organelle containing their cognate DNA triggers.

TL;DR: The capabilities of the current cell-free platforms for executing DNA programs in vitro are reviewed and the recent advances in programming usingcell-free expression are described, a multidisciplinary playground that has enabled a myriad of novel applications in synthetic biology, biotechnology, and biological physics.

TL;DR: 曼氏血吸虫感染后，宿主活化CD4^＋Th2细胞Ｌ分泌IL-4、IL-5和 IL-13。

TL;DR: A protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity was developed, and omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

TL;DR: It is demonstrated the linkage of genotype to phenotype in man-made compartments using a model system and a selection for target-specific DNA methylation was based on the resistance of the product (methylated DNA) to restriction digestion.

TL;DR: The alpha-hemolysin pore protein from Staphylococcus aureus is expressed inside the vesicle to create a selective permeability for nutrients to solve the energy and material limitations and increase the capacity of the reactor.