Journal Article10.1079/BJN19960129
In vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds
TL;DR: The characteristic fermentation pattern of the total fibres from the three brown algae investigated was attributed to the peculiar fermentation of alginates, and mannuronate was shown not to be directly involved.
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Abstract: The in vitro degradation of dietary fibre from three brown seaweeds (Himanthalia elongata, Laminaria digitata and Undaria pinnatifida) was studied, using human faecal flora. Two sets of fibre were tested: (1) total algal fibres extracted from the whole algae, mainly composed of alginates, and (2) purified fibres (sulphated fucans, Na-alginates and laminarans) representative of those contained in the whole brown algae. Mannuronate, one algal component, was also investigated. Substrate disappearance and short-chain fatty acid (SCFA) production were monitored after 6, 12 and 24 h fermentation. Gas production was followed hourly during the first 9 h and then at 12 and 24 h. Sugarbeet fibre was used as a fermentation reference substrate. According to the fermentative indices used, most of each of the total algal fibres disappeared after 24 h (range 60-76%) but, unlike the reference substrate, they were not completely metabolized to SCFA (range 47-62%). Among the purified algal fibres, disappearance of laminarans was approximately 90% and metabolism to SCFA was approximately 85% in close agreement with the fermentation pattern of reference fibres. Sulphated fucans were not degraded. Na-alginates exhibited a fermentation pattern quite similar to those of the whole algal fibres with a more pronounced discrepancy between disappearance and production of SCFA: disappearance was approximately 83% but metabolism was only approximately 57%. Mannuronate was slowly fermented but its metabolism corresponded to its disappearance from the fermentative medium. Thus, the characteristic fermentation pattern of the total fibres from the three brown algae investigated was attributed to the peculiar fermentation of alginates, and mannuronate was shown not to be directly involved.
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Abstract: Many separation methods are based on chromatography, that is, separation of the components of a mixture by differences in the way they become distributed (or partitioned) between two different phases. To illustrate with an extreme example, suppose we have a mixture of gaseous methane and ammonia and contact this mixture with water. Ammonia, being very soluble in water (~\(90 \: \text{g}\) per \(100 \: \text{g}\) water at \(1 \: \text{atm}\) pressure), will mostly go into the water phase, whereas the methane, being almost insoluble (~\(0.003 \: \text{g}\) per \(100 \: \text{g}\) of water) will essentially remain entirely in the gas phase. Such a separation of methane and ammonia would be a onestage partitioning between gas and liquid phases and, clearly, could be made much more efficient by contacting the gas layer repeatedly with fresh water. Carried through many separate operations, this partitioning procedure is, at best, a tedious process, especially if the compounds to be separated are similar in their distributions between the phases. However, partitioning can be achieved nearly automatically by using chromatographic columns, which permit a stationary phase to be contacted by a moving phase. To illustrate, suppose a sample of a gaseous mixture of ammonia and methane is injected into a long tube (column) filled with glass beads moistened with water (the stationary phase), and a slow stream of an inert carrier gas, such as nitrogen or helium, is passed in to push the other gases through. A multistage partitioning would occur as the ammonia dissolves in the water and the resulting gas stream encounters fresh water as it moves along the column. Carrier gas enriched with methane would emerge first and effluent gas containing ammonia would come out later. This is a crude description of the method of gas-liquid chromatography (abbreviated often as glc, GC, or called vapor-phase chromatography, vpc). This technique has become so efficient as to revolutionize the analysis and separation of almost any organic substance that has even a slight degree of volatility at some reasonably attainable temperature. The most modern glc equipment runs wholly under computer control, with preprogrammed temperatures and digital integration of the detector output. A wide variety of schemes is available for measuring the concentration of materials in the effluent carrier gas, and some of these are of such extraordinary sensitivity that only very small samples are necessary (\(10^{-9} \: \text{g}\), or less).
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Seaweed: Chemical composition and potential food uses
Keiji Ito,Kanji Hori +1 more
TL;DR: In this article, the chemical components of seaweeds are reviewed in relation to potential food uses, and the nutritive values of seaweed are briefly discussed, and practical uses for seaweeds as food in Japan are described.
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