Journal Article10.1016/j.bioadv.2024.213873
In vitro development of a muscle-tendon junction construct using decellularised extracellular matrix: Effect of cyclic tensile loading
Nagakute Iwasaki,Marta Roldo,Aikaterina Karali,Gordon Blunn +3 more
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TL;DR: In vitro development of a muscle-tendon junction construct using decellularised extracellular matrix: Effect of cyclic tensile loading - Decellularised extracellular matrix (DECM) derived from the muscle tendon junction (MTJ) can induce MTJ marker gene and protein expression by human mesenchymal stem cells (MSCs).
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Abstract: The muscle tendon junction (MTJ) plays a crucial role in transmitting the force generated by muscles to the tendon and then to the bone. Injuries such as tears and strains frequently happen at the MTJ, where the regenerative process is limited due to poor vascularization and the complex structure of the tissue. Current solutions for a complete tear at the MTJ have not been successful and therefore, the development of a tissue-engineered MTJ may provide a more effective treatment. In this study, decellularised extracellular matrix (DECM) derived from sheep MTJ was used as a scaffold in order to provide a scaffold for the MTJ with the relevant mechanical properties and differentiation cues such as growth factors. Human mesenchymal stem cells (MSCs) were seeded on DECM and 10 % cyclic strain was applied using a bioreactor. MSCs cultured on DECM showed significantly higher gene and protein expression of MTJ markers such as collagen 22, paxillin and talin, than MSCs in 2D culture. Although collagen 22 protein expression was higher in the cells with strain than without strain, reduced gene expression of other MTJ markers was observed when the strain was applied. DECM combined with 10 % strain enhanced myogenic differentiation, while tenogenic differentiation was reduced when compared to static cultures of MSCs on DECM. For the first time, these results showed that DECM derived from the MTJ can induce MTJ marker gene and protein expression by MSCs, however, the effect of strain on the MTJ development in DECM culture needs further investigation.
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Citations
Tendon Tissue Engineering: Pathophysiological Mechanism and Bioengineering Therapy of Tendinopathy
Bin Chen,Yingqi Zhao,Shizhen Guo,Chuyue Tang,Baoyun Xu,Mei Zhou,Qianbo Chen,Lin Ma,Jingtong Lyu,Lin Guo,Yunjiao Wang,Bin Chen,Yingqi Zhao,Shizhen Guo,Chuyue Tang,Baoyun Xu,Mei Zhou,Qianbo Chen,Lin Ma,Jingtong Lyu,Lin Guo,Yunjiao Wang +21 more
Abstract: Tendinopathy afflicts many professional athletes and the elderly. However, due to the unique cellular and histological composition of tendons, healing is frequently unsatisfactory. The clinical physical therapy and surgical interventions often fail to meet patient expectations. In recent years, bioengineering technology has undergone rapid development, with a significant number of studies in the biological field focusing on bioengineering technology to explore emerging treatments for diseases. Therefore, bioengineering technology has the potential to become an important part of future tendon healing therapies. The present article will describe the sources of scaffolds, biological factors and bioengineering strategies, with a focus on their current applications in laboratory and clinical contexts.
Trifecta of Tendon Regeneration: 3D Bioprinted Scaffolds Recapitulate Hierarchical Interfaces From Muscle‐to‐Bone
Xuemiao Liu,Ying Cen,Weiguo Zhang,Kang Tian,Fu-Zhen Yuan,Xing Wang +5 more
Abstract: Abstract Functional tendon regeneration faces dual challenges: limited self‐healing capacity and the complexity of three hierarchical interfaces—the myotendinous junction (MTJ), tendon proper, and bone‐tendon junction (BTJ). Conventional repair strategies often fail to address these critical barriers, resulting in a higher probability of re‐tear after the surgery. 3D bioprinting emerges as a transformative approach, enabling recapitulation of these multiscale interfaces through precise structural design, functional material gradients, and bioactive integration. This review comprehensively analyzes recent advances in bioprinted tendon scaffolds, focusing on tripartite structural‐biological requirements across MTJ, tendon proper, and BTJ microenvironments. Critical examination is given to the synergistic regulation of bioprinting technologies and material diversity for replicating native mechanobiological cues. Furthermore, innovative scaffold design strategies target each interface's unique regeneration challenges: anisotropic muscle‐tendon integration, load‐bearing tendon remodeling, and mineralized osteotendinous regeneration. Finally, translational roadblocks and future directions are assessed, emphasizing in vivo functional reintegration of the muscle‐to‐bone continuum and scalable manufacturing for clinical adoption.
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Effect of uniaxial stretching on rat bone mesenchymal stem cell: Orientation and expressions of collagen types I and III and tenascin-C
TL;DR: Cyclic stretching promotes the synthesis of collagen types I and III and tenascin‐C by the rat BMSC, suggesting that mechanical forces regulate the biological function of cells.