Improved dna amplification method

TL;DR: In this paper, a thermal cycling method and device is described, which comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures such as the DNA polymerase chain reaction.

TL;DR: In this article, temperature uniformity and time constant measurements of an 18-vessel array using encapsulated liquid crystals suspended in the liquid were obtained by measuring the temperature distribution in the reacting liquid.

TL;DR: In this paper, a method is described for analyzing a sample for the presence of a nucleic acid wherein the sample is amplified, illustratively using PCR, and the fluorescence at a selected amplification cycle is compared to the baseline region to determine whether the measurement falls outside of that baseline region.

TL;DR: The data indicate that amplification efficiencies, especially in the first cycles, depend not only on the priming efficiencies of the primers and the melting temperature of the Amplicon, but also on the melting behavior of the amplicon's genomic vicinity.