Journal Article10.1093/CLINCHEM/34.9.1853
Improved agarose electrophoretic method for separating alkaline phosphatase isoenzymes in serum.
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TL;DR: Because the electrophoretic mobilities of this "intestinal variant" and of some immunoglobulin-bound ALP fractions are identical to those of bone and intestinal ALP, respectively, treatment of the samples with a polyclonal antibody that reacts with intestinal ALP is advised.
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Abstract: A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Bone, liver, high-molecular-mass, and intestinal ALP are separated with high reproducibility. The sensitivity of the agarose system is superior to cellulose acetate in detecting high-Mr ALP. Correlation is good between bone ALP fractions scanned before and after treatment with neuraminidase. Immunoglobulin-bound ALPs, the ALP-lipoprotein-X complex, and the additional ALP fraction observed in transient hyperphosphatasemia in children are detected by their peculiar electrophoretic mobility in the proposed system. Approximately 25% of the samples contained an additional fraction of intestinal-type ALP, as evidenced by neuraminidase treatment and use of polyclonal and monoclonal antibodies. Because the electrophoretic mobilities of this "intestinal variant" and of some immunoglobulin-bound ALP fractions are identical to those of bone and intestinal ALP, respectively, treatment of the samples with a polyclonal antibody that reacts with intestinal ALP is advised.
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References
Recommended Methods for the Determination of Four Enzymes in Blood
R. Keiding,Mogens Hørder,W. Gerhardt Denmark,E. Pitkänen,R. Tenhunen,Johan H. Strømme,Liv Theodorsen,J. Waldenström,N. Tryding,L. Westlund +9 more
TL;DR: The results obtained in this study allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for humans and other animals.
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•Journal Article
A Serum Alkaline Phosphatase Isoenzyme of Human Neoplastic Cell Origin
TL;DR: The possibility that elevated serum alkaline phosphatase in patients with metastatic cancer can be of neoplastic rather than of hepatic and osseous origin is demonstrated.
372
Alkaline phosphatase isoenzymes.
TL;DR: The aim of future research should be to remove the limitations at present imposed on quantitative analysis by the close similarities of bone and liver alkaline phosphatases.
320
Molecular cloning and sequence analysis of human placental alkaline phosphatase.
TL;DR: The complete amino acid sequence of the precursor and mature forms of human placental alkaline phosphatase have been inferred from analysis of a cDNA.
295
Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma.
S B Rosalki,A Y Foo +1 more
TL;DR: Two new methods for the separation and quantification of the bone and liver isoenzymes of alkaline phosphatase (EC 3.3.1.1) in plasma are described, rapid, reproducible, and suitable for use in the diagnostic laboratory.
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