Journal Article10.1021/JF073127H
Immunostimulating Activity of a Crude Polysaccharide Derived from Green Tea (Camellia sinensis) Extract
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TL;DR: The catechin-polysaccharide complex is a very important molecule in the immunomodulating activity of tea extracts, and its activities were dependent on the content of strictinin in the leaf extract.
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Abstract: Green tea extract is well-known to reduce the risk of a variety of diseases. Here, we investigated the immunostimulating activity of tea polysaccharide (TPS), one of the main components in green tea extract. The water extracts from mature or immature tea leaves were precipitated by using ethanol at room temperature. The sediment was washed with ethanol and acetone alternately and then dried. We used the phagocytic activity of macrophage-like cells as an indicator of immune function activation. Chemical components were analyzed by HPLC. The immunostimulating activity of TPS from immature leaves extract was higher than that of TPS from mature leaves, and its activities were dependent on the content of strictinin in the leaf extract. Futhermore, a mixture of catechin and TPS that removed polyphenols did not increase the immunostimulating activity. These results suggest that the catechin-polysaccharide complex is a very important molecule in the immunomodulating activity of tea extracts.
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Citations
A review on the isolation and structure of tea polysaccharides and their bioactivities
TL;DR: In this article, the various aspects of the investigation results of tea polysaccharides in the past 20 years were summarized, including extraction and purification processes, the structure and bioactivities.
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Metabolomics Analysis Reveals the Compositional Differences of Shade Grown Tea (Camellia sinensis L.)
Kang-Mo Ku,Jung Nam Choi,Jiyoung Kim,Jeong Kee Kim,Lang Gook Yoo,Sang Jun Lee,Young-Shick Hong,Choong Hwan Lee +7 more
TL;DR: The metabolomic approaches are highlighted to find out the effect of cultivation methods on chemical composition in plants and the relationship with antioxidant activity and the possibility to get high umami and less astringent green teas in shade culture is delineated.
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Recent advances in tea polysaccharides: Extraction, purification, physicochemical characterization and bioactivities.
TL;DR: In present review, the recent literature, update the information and put forward future perspectives on TPS covering its extraction, purification, quantitative determination techniques as well as physicochemical characterization and bioactivities are summarized.
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Sugar compositions, α-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods
TL;DR: It seemed that enzyme extraction could be more conducive to increase the content of Ara, Gal and GaLA, and the molecular weights of TFPS were larger than those of TLPS, while those of polysaccharides obtained by EE decreased.
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Preparation, preliminary characterization, antioxidant, hepatoprotective and antitumor activities of polysaccharides from the flower of tea plant (Camellia sinensis).
TL;DR: TFPS should be a potent natural polymer with antioxidant, hepatoprotective and antitumor activities, as suggested by the results of the present study.
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References
Changes in Epigallocatechin-3-O-(3-O-methyl) Gallate and Strictinin Contents of Tea (Camellia sinensis L.) Cultivar ‘Benifuki’ in Various Degrees of Maturity and Leaf Order
Mari Maeda-Yamamoto,Hiroshi Nagai,Kazumi Asai,Sawako Moriwaki,Hideki Horie,Katsunori Kohata,Hirofumi Tachibana,Toshio Miyase,Mitsuaki Sano +8 more
TL;DR: To obtain the anti-allergic benefits of EGCG3″Me, green tea should be made from fully-matured ‘Benifuki’ leaves under the fourth shoot late in the first crop or under the third shoot in the second crop, refined by removing stems.
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Determination of Mechanism of Flock Sediment Formation in Tea Beverages
TL;DR: The HP-20 adsorbed fraction contained little strictinin and formed hardly any sediment, suggesting that control of the stricterinin content is significant in avoiding sediment formation during the manufacturing process of tea beverages.
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Antitumor-Promoting Effects of Tea Aqueous Nondialysates in Mouse Epidermal JB6 Cells
Yoshiyuki Nakamura,Ichiro Kawase,Seiji Harada,Michiaki Matsuda,Takeshi Honma,Isao Tomita +5 more
- 01 Jan 1997
TL;DR: In this article, a new antitumor-promoting component, tea aqueous nondialysates (TNDs), was reported, which was prepared from hot water infusion of green and black tea leaves followed by extraction with CHC13, ethyl acetate, and n-butanol.
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Technique for screening immune-enhancing polysaccharides in food using 1,25-dihydroxyvitamin D3-differentiated HL60 cells.
TL;DR: A technique for screening immune-enhancing polysaccharides in food using the phagocytotic activity of 1,25-dihydroxyvitamin D3 (VD3)-differentiated HL60 cells is presented.
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