Journal Article10.1002/arch.22130
Immunological function of Bombyx Toll9-2 in the silkworm (Bombyx mori) larval midgut: Activation by Escherichia coli/lipopolysaccharide and regulation of growth.
Jisheng Liu,Weifeng Yang,Wenli Liao,Weijian Chen,Xiaoling Bu,Shiyi Huang,Wanyi Jiang,Luc Swevers +7 more
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TL;DR: This study investigates the immunological function of Bombyx Toll9-2 in silkworm larvae, revealing its role as a pathogen-associated receptor responsive to Escherichia coli/lipopolysaccharide, and its regulation of growth and immune response in the midgut.
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Abstract: Toll receptors are important regulators of insects' innate immune system which, upon binding of pathogen molecules, activate a conserved signal transduction cascade known as the Toll pathway. RNA interference (RNAi) is a powerful tool to study the function of genes via reverse genetics. However, due to the reported refractory of RNAi efficiency in lepidopteran insects, successful reports of silencing of Toll receptors in the silkworm Bombyx mori have not been reported yet. In this study, a Toll receptor of the silkworm Bombyx Toll9-2 (BmToll9-2) was cloned and its expression and function were analyzed. The results showed that BmToll9-2 contains an ectodomain (ECD) with a signal peptide and nine leucine-rich repeats, a transmembrane helix, and a cytoplasmic region with a Toll/interleukin-1 domain. Phylogenetic analysis indicates that BmToll9-2 clusters with other insect Toll9 receptors and mammalian Toll-like receptor 4. Oral infection of exogenous pathogens showed that the Gram-negative bacterium Escherichia coli and its main cell wall component lipopolysaccharide (LPS), as well as the Gram-positive bacterium Staphylococcus aureus and its main cell wall component peptidoglycan, significantly induce BmToll9-2 expression in vivo. LPS also induced the expression of BmToll9-2 in BmN4 cells in vitro. These observations indicate its role as a sensor in the innate immunity to exogenous pathogens and as a pathogen-associated receptor that is responsive to LPS. RNAi of BmToll9-2 was effective in the midgut and epidermis. RNAi-mediated knock-down of BmToll9-2 reduced the weight and growth of the silkworm. Bacterial challenge following RNAi upregulated the expression of BmToll9-2 and rescued the weight differences of the silkworm, which may be related to its participation in the immune response and the regulation of the microbiota in the midgut lumen of the silkworm larvae.
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Citations
BmToll9-1 Is a Positive Regulator of the Immune Response in the Silkworm Bombyx mori
Jisheng Liu,Weijian Chen,Jinrong Situ,Jiaxuan Li,Jiahua Chen,Minchun Lai,Fengyi Huang,Baoqi Li +7 more
TL;DR: BmToll9-1 is a positive regulator of the immune response in Bombyx mori, acting beyond its receptor function, with expression profiles indicating its involvement in modulating the Toll pathway to enhance innate immunity against pathogens.
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