Journal Article10.1002/EJI.1830121102
Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. V. H-2 associativity of variant-specific antigens.
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TL;DR: By in vitro mutagenesis of mastocytoma P815, it is possible to obtain tumor cell variants that are rejected by syngeneic mice (tum−), and most of these variants carry new individual antigens and stimulate a specific cytolytic T cell response in mixed leukocyte tumor cell culture (MLTC).
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Abstract: By in vitro mutagenesis of mastocytoma P815, it is possible to obtain tumor cell variants that are rejected by syngeneic mice (tum−). Most of these variants carry new individual antigens and stimulate a specific cytolytic T cell (CTL) response in mixed leukocyte tumor cell culture (MLTC). The H-2 associativity of this response was examined for six different variants by measuring the inhibition of cell-mediated cytolysis by antibodies directed against products of the K or the D end of the H−2d complex. The lysis was either not inhibited (variants P91 and PI 16) or inhibited selectively by anti-Kd (variants P21, P32 and P198) or anti-Dd antibodies (variant P35). All these tum− variants expressed Kd and Dd antigens as measured by absorption of H-2 alloantisera.
Long-term CTL clones can be obtained that are specific for individual turn antigens. The pattern of H-2 associativity obtained with MLTC-derived CTL against four tum− variants was verified with CTL clones directed against the specific antigens of these variants. Concordant results were observed in all cases. In addition to CTL clones specific for tum− antigens, it is possible to isolate clones against a P815 tumor-associated antigen found on all P815 tum− variants. For these clones no clear associativity with either Kd or Dd products was found.
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Citations
Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells.
Christophe Lurquin,Christophe Lurquin,Aline Van Pel,Aline Van Pel,Bernard Mariamé,Bernard Mariamé,Etienne De Plaen,Etienne De Plaen,Jean-Pierre Szikora,Jean-Pierre Szikora,Catherine Janssens,Catherine Janssens,Matthias J. Reddehase,Joseph Lejeune,Joseph Lejeune,Thierry Boon,Thierry Boon +16 more
TL;DR: The gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence, which creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule.
316
Toward a Genetic Analysis of Tumor Rejection Antigens
TL;DR: The chapter focuses on the possibility of using a direct genetic approach to identify tumor rejection antigens encoded by the cellular genome, and describes the analysis of the genes coding for these antigen and its implications for T cellmediated immune surveillance.
248
Antigenic Tumor Cell Variants Obtained with Mutagens
TL;DR: Evidence that mutagenic treatment may represent an additional way to increase the immunogenicity of tumor cells is reviewed, suggesting that significant tumor inhibition may be obtained by specific removal of the relevant suppressor T cells by anti-idiotypic immunization.
168
Structure of the gene of tum- transplantation antigen P198: a point mutation generates a new antigenic peptide.
C Sibille,Patrick Chomez,Claude Wildmann,A Van Pel,E De Plaen,Janet L. Maryanski,V de Bergeyck,Thierry Boon +7 more
TL;DR: It is concluded that tum- mutation P198 generates a new epitope recognized by syngeneic T cells that is able to compete for binding to major histocompatibility complex molecule Kd.
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Immunogenic (tum-) variants obtained by mutagenesis of mouse mastocytoma P815. VIII. Detection of stable transfectants expressing a tum- antigen with a cytolytic T cell stimulation assay.
Thomas Wölfel,Thomas Wölfel,Aline Van Pel,Aline Van Pel,Etienne De Plaen,Etienne De Plaen,Christophe Lurquin,Christophe Lurquin,Janet L. Maryanski,Janet L. Maryanski,Thierry Boon,Thierry Boon +11 more
TL;DR: In this article, the authors used DNA from tum− variant P91 mixed with a plasmid carrying an antibiotic resistance gene, at a frequency of approximately 1 in 13 000 antibiotic-resistant transfectants.
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