Identification of molecularly defined Staphylococcus aureus strains using matrix-assisted laser desorption/ionization time of flight mass spectrometry and the Biotyper 2.0 database
TL;DR: This first study where the Biotyper 2.0 database has been applied for bacterial identification in a local strain collection of molecularly defined Staphylococcus aureus shows that the accuracy of the MALDI-TOF MS-based S. a Aureus identification approach was clearly faster than more time-consuming methods such as a molecular identification approach.
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Abstract: Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced for bacterial identification. To our knowledge, this is the first study where the Biotyper 2.0 database (Bruker Daltonics) has been applied for bacterial identification in a local strain collection of molecularly defined Staphylococcus aureus. We showed that the accuracy of the Biotyper 2.0-based identification for 602 molecularly defined strains of S. aureus, irrespective of meticillin resistance, was equivalent to that of the molecularly defined reference even at a score cut-off value of 2. Also, 412 isolates of 20 different species of non-S. aureus staphylococci were all correctly identified to species level compared to the molecularly defined reference. Moreover, the MALDI-TOF MS-based S. aureus identification approach was clearly faster than more time-consuming methods such as a molecular identification approach.
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Citations
Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology
TL;DR: This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MalDI-toF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy.
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TL;DR: Strain-level resolution has been reported with diverse bacteria, using library-based and bioinformatics-enabled approaches, and recent data suggest that these limits might not yet have been reached.
MALDI-TOF Mass Spectrometry for Microorganism Identification
TL;DR: This article summarizes the current literature on MALDI-TOF MS for microbial identification and provides a preview of the method's potential future applications in clinical microbiology.
226
Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram-Negative Bacilli
Ryan T. Saffert,Scott A. Cunningham,Sherry M. Ihde,Kristine E. Monson Jobe,Jayawant N. Mandrekar,Robin Patel +5 more
TL;DR: The Bruker Biotyper overall outperformed the BD Phoenix for identification of Gram-negative bacilli to the genus (P < 0.0001) and species (P = 0.0005) level in this sample set.
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Coagulase-negative staphylococci: update on the molecular epidemiology and clinical presentation, with a focus on Staphylococcus epidermidis and Staphylococcus saprophyticus
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167
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