Journal Article10.1007/S00122-021-03903-1
Identification of genomic loci conferring broad-spectrum resistance to multiple nematode species in exotic soybean accession PI 567305.
Tri D. Vuong,Humira Sonah,Gunvant Patil,C. Meinhardt,Mariola Usovsky,Ki-Seung Kim,Ki-Seung Kim,François Belzile,Z. Li,Robert T. Robbins,J. G. Shannon,Henry T. Nguyen +11 more
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TL;DR: In this paper, a bi-parental population consisting of 242 F8-derived recombinant inbred lines (RILs) was developed from a cross of a nematode susceptible cultivar, Magellan, and resistant accession, PI 567305.
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Abstract: Genetic analysis identified a unique combination of major QTL for resistance to important soybean nematodes concurrently present in a single soybean accession, which has not been reported earlier. An exotic soybean [Glycine max (L.) Merr.] accession, PI 567305, was reported to be highly resistant to three important nematode species, soybean cyst (SCN), root-knot (RKN), and reniform (RN) nematodes. However, genetic basis controlling broad-spectrum resistance in this germplasm has not been investigated. We report results of genetic analysis to identify genomic loci conferring resistance to these nematode species. A bi-parental population consisting of 242 F8-derived recombinant inbred lines (RILs) was developed from a cross of a nematode susceptible cultivar, Magellan, and resistant accession, PI 567305. The RILs were phenotyped for nematode resistance to three SCN HG types. They were genotyped using the Infinium SoySNP6K BeadChips and genotype-by-sequencing (GBS) methods in an attempt to evaluate the cost-effectiveness and efficiency of these two genotyping platforms. Genetic analysis confirmed the major QTL on chromosomes (Chrs) 10 and 18 with broad-spectrum resistance to the three nematodes present in this germplasm. Haplotype and copy number variation analyses of SCN resistance QTL indicated that PI 567305 has a different haplotype, which is associated with likely a unique SCN resistance mechanism different from Peking- or PI 88788-type resistance. The evaluations of both Infinium Beadchip- and GBS-based genotyping technologies provided comprehensive insights for researchers to choose a cost-effective and efficient platform for QTL mapping and for other genomic studies in soybeans.
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Citations
Breeding for disease resistance in soybean: a global perspective
Feng Lin,Sushil Satish Chhapekar,C. C. Vieira,Marcos Paulo da Silva,Alejandro Rojas,Dongho Lee,Nianxi Liu,Esteban Mariano Pardo,Yi-Chen Lee,Zhi-min Dong,José Baldin Pinheiro,Leonardo Daniel Ploper,John C. Rupe,Pengyin Chen,Dechun Wang,Henry T. Nguyen +15 more
TL;DR: In this paper , a comprehensive atlas of resistant genes, genes, and alleles for 28 soybean diseases was provided. But, the authors focused on the most important soybean disease atlas, where the authors provided a comprehensive summary of important resistant genes/alleles.
Molecular Breeding to Overcome Biotic Stresses in Soybean: Update
TL;DR: A review of genetic aspects of various diseases caused by pathogens in soybean and molecular breeding research work conducted to date is presented in this paper , where the main objectives of soybean crop improvement are based on the identification of sources of resistance or tolerance against various biotic as well as abiotic stresses and utilization of these sources for further hybridization and transgenic processes for development of new cultivars for stress management.
Exploring Soybean Resistance to Soybean Cyst Nematode.
TL;DR: In this paper , the soybean cyst nematode (SCN) resistance to quantitative trait loci (QTLs) has been investigated in the past decade and significant advances have been made.
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3D-GBS: a universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes
TL;DR: The 3D-GBS as mentioned in this paper protocol uses a combination of three restriction enzymes (PstI/NsiI/MspI) to reduce the amount of missing data and provide a more uniform distribution of SNPs across the genome.
Cataloging SCN resistance loci in North American public soybean breeding programs
Anser Mahmood,Kristin D Bilyeu,Mária Škrabišová,Jana Biová,Elizabeth J. De Meyer,C. Meinhardt,Mariola Usovsky,Qijian Song,Aaron J. Lorenz,Melissa G. Mitchum,Grover Shannon,Andrew Scaboo +11 more
TL;DR: The genetic landscape of SCN resistance loci in North American public soybean breeding programs reveals seven major genetic loci, including three novel loci, impacting resistance to multiple SCN populations.
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