Identification of differentially methylated genes in the malignant transformation of ovarian endometriosis
TL;DR: RassF2, RUNX3, GSTZ1, CYP2A, GBGT1, NDUFS1, SPOCK2, ADAM22, and TRIM36 were candidate genes for malignant transformation of ovarian EMS and epigenetic inactivation of RASSF2 by promoter hypermethylation is an early event in malignant transformed ovarian EMS.
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Abstract: Key roles for epigenetic mechanisms in tumorigenesis are well accepted, while the relationship between gene methylation and malignant transformation of ovarian endometriosis (EMS) was seldom reported. In this study, we aimed to screen for aberrantly methylated genes associated with the malignant transformation of ovarian EMS and to preliminarily verify the reliability of screened results by detecting the methylation status and protein expression of the candidate gene in a larger scale of formaldehyde-fixed and paraffin-embedded (FFPE) samples. Methylated CpG island amplification coupled with representational difference analysis (MCA-RDA) was performed on 3 couples of endometriosis-associated ovarian carcinoma (EAOC) fresh samples to identify differentially methylated candidate genes related to malignant transformation of ovarian EMS; Methylation-specific PCR (MSP) and immunohistochemistry were performed in 30 EAOC samples to detected the methylation status and protein expression of RASSF2 gene to verify the reliability of MCA-RDA results. Nine differentially methylated genes were obtained by MCA-RDA as candidate genes for malignant transformation of EMS; Methylation frequency of RASSF2 in the neoplastic tissues of EAOC group was higher than that in the ectopic endometria (p < 0.05). While protein expression of RASSF2 in the neoplastic tissues was lower than that in the ectopic endometria of the EAOC group (p < 0.05) Absence of protein expression of RASSF2 was significantly correlated with the promoter methylation of the gene (p < 0.05). RASSF2, RUNX3, GSTZ1, CYP2A, GBGT1, NDUFS1, SPOCK2, ADAM22, and TRIM36 were candidate genes for malignant transformation of ovarian EMS and epigenetic inactivation of RASSF2 by promoter hypermethylation is an early event in malignant transformation of ovarian EMS. The screen results were reliable and worthy of further study.
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Citations
m6A methylation of RNF43 inhibits the progression of endometriosis through regulating oxidative phosphorylation via NDUFS1
Yuxin Tang,Xiaotang Lu,Kexin Lin,Jiayi Li,Ming Yuan,Kaiqing Lin +5 more
TL;DR: It is found RNF43 was downregulated in ectopic endometrial tissue and primary ectopic endometrial stromal cells (ECESCs), and the expression and stability of RNF43 mRNA were regulated by the Methyltransferase-like 3 (METTL3)/IGF2BP2 m6A modification axis.
SPOCK2 Affects the Biological Behavior of Endometrial Cancer Cells by Regulation of MT1-MMP and MMP2.
TL;DR: There was significantly less SPOCK2 protein expression in the EC tissue than in the normal endometrium tissue, and lack of SPock2protein expression in EC tissue was associated with distant metastasis and myometrial invasion.
MiR-3130-5p is an intermediate modulator of 2q33 and influences the invasiveness of lung adenocarcinoma by targeting NDUFS1.
TL;DR: In this paper, miR-3130-5p directly regulates the expression of NDUFS1 and the corresponding tumor invasiveness, migration and epithelial-mesenchymal transition (EMT).
New insights about endometriosis-associated ovarian cancer: pathogenesis, risk factors, prediction and diagnosis and treatment
Biqing Chen,Liping Zhao,Rulin Yang,Tianmin Xu +3 more
TL;DR: A comprehensive survey of the current progress in comprehending the pathogenesis of malignant transformation in endometriosis is presented, which examines the most recent research discoveries concerning the diagnosis of EAOC and emphasizes potential targets for therapeutic intervention.
An update on endometriosis biomarkers.
Kyle N Le,Camran Nezhat,Ceana Nezhat,Ariel Benor,Alan Decherney +4 more
TL;DR: There are emerging data on the utility of microRNA for diagnosis and prognosis of disease activity and there are emerging data on the utility of microRNA for diagnosis and prognosis of disease activity for endometriosis.
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