Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana1
TL;DR: This work used a polymerase chain reaction‐based strategy to isolate a cDNA, designated IgASE1, encoding a polyunsaturated fatty acid‐elongating activity from I. galbana, the first time such an elongating activity has been functionally characterised.
read more
About: This article is published in FEBS Letters. The article was published on 16 Jan 2002. and is currently open access. The article focuses on the topics: Linoleic acid & Docosahexaenoic acid.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants.
TL;DR: The prospect of producing this important polyunsaturated fatty acid in transgenic oilseeds is highlighted, as is the urgent need for a sustainable replacement for diminishing fish stocks.
232
Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis.
Astrid Meyer,Helene Kirsch,Frédéric Domergue,Amine Abbadi,Petra Sperling,Joerg Bauer,Petra Cirpus,Thorsten Zank,Hervé Moreau,Thomas J. Roscoe,Ulrich Zähringer,Ernst Heinz +11 more
TL;DR: DHA synthesis in stearidonic acid-fed yeast is successfully implemented by coexpression of the Δ6- and Δ5-elongases from T. tauri and O. pseudonana with the Δ5 and Δ4-desaturases from two other algae.
229
LC-PUFA from photosynthetic microalgae: occurrence, biosynthesis, and prospects in biotechnology
TL;DR: This mini-review presents an introductory overview of LC- PUFA-related health benefits in humans, describes LC-PUFA occurrence in diverse microalgal classes, depicts the major pathways of their biosynthesis in microalgae, and discusses the prospects for micro algal LC-FUFA production.
209
Identification of two novel microalgal enzymes involved in the conversion of the ω3-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid
TL;DR: Yeast co-expression studies, with pavELO and IgD4, revealed that these genes were capable of functioning together to carry out the two-step conversion of EPA into DHA.
Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants
TL;DR: The application of new technologies, for example lipidomics and next-generation sequencing, is providing a better understanding of seed oil biosynthesis and opportunities for increasing the production of unusual fatty acids.
References
Studies of marine planktonic diatoms: i. cyclotella nana hustedt, and detonula confervacea (cleve) gran.
TL;DR: Bacteria-free clones of the small centric diatom Cyclotella nana Hustedt were isolated, three from estuarine localities, one from Continental Shelf waters, and one from the Sargasso Sea as mentioned in this paper.
7.7K
Desaturation and related modifications of fatty acids1
John Shanklin,Edgar B. Cahoon +1 more
- 01 Jun 1998
TL;DR: Biochemical and structural similarities between the integral membrane enzymes suggest that this class also uses a diiron cluster for catalysis, and it is anticipated that rational design of these enzymes will result in new and desired activities that may form the basis for improved oil crops.
Cloning, Expression, and Nutritional Regulation of the Mammalian Δ-6 Desaturase
TL;DR: Northern analysis revealed that the brain contained an amount of Δ-6 desaturase mRNA that was several times greater than that found in other tissues including the liver, lung, heart, and skeletal muscle.
668
A 5-bp deletion in ELOVL4 is associated with two related forms of autosomal dominant macular dystrophy
Kang Zhang,Kang Zhang,Marina Kniazeva,Min Han,Wen Li,Zhengya Yu,Zhenglin Yang,Yang Li,Michael L. Metzker,Rando Allikmets,Donald J. Zack,Laura E. Kakuk,Pamela S. Lagali,Paul Wong,Ian M. MacDonald,Paul A. Sieving,David J. Figueroa,Christopher P. Austin,Robert J. Gould,Radha Ayyagari,Konstantin Petrukhin +20 more
TL;DR: These results are the first to implicate the biosynthesis of fatty acids in the pathogenesis of inherited macular degeneration and identify a single 5-bp deletion within the protein-coding region of a new retinal photoreceptor-specific gene, ELOVL4, in all affected members of STGD3 and adMD families.
470