Open Access
Identification and characterization of miRNA expression during zebrafish retinal regeneration
Rachel L. Harding
- 28 Mar 2014
6
TL;DR: Dicer knockdown prior to light damage lead to decreased proliferation in regenerating retinas without affecting the number of Müller glia during retinal regeneration or cell death.
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Abstract: by Rachel Harding Unlike most other vertebrates, zebrafish are able to regenerate many tissues including retina. Light-induced photoreceptor cell death causes inner nuclear layer Müller glia to dedifferentiate, reenter the cell cycle and produce proliferating progenitor cells. These progenitor cells continue to proliferate and migrate to the appropriate retinal layer and differentiate into functional photoreceptors. While a number of genes essential to retinal regeneration have been identified, the mechanisms regulating these genes have been little studied. Many post-transcriptional regulatory mechanisms exist in the cell including miRNAs, short RNA sequences encoded in the genome. To determine if miRNAs play a role in retinal regeneration, I reduced global miRNA expression by Dicer knockdown, an enzyme essential to miRNA maturation. Dicer knockdown prior to light damage lead to decreased proliferation in regenerating retinas without affecting the number of Müller glia during retinal regeneration or cell death. These studies demonstrate an essential role
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MicroRNAs: Target Recognition and Regulatory Functions
David P. Bartel
- 01 Jan 2009
TL;DR: In this article, a review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.
656
•Dissertation
Development and regeneration of neuronal circuits in the vertebrate retina
Florence DeEtte D'Orazi
- 22 Sep 2016
TL;DR: Development and regeneration of neuronal circuits in the vertebrate retina and the role of ‘spiking’ in the regeneration of these circuits is studied.
4
References
Small but influential: the role of microRNAs on gene regulatory network and 3′UTR evolution
Rui Zhang,Bing Su +1 more
TL;DR: This work focuses on the post-transcriptional level gene regulation of miRNAs in animals and reviews how the mi RNAs act to sustain and shape up the expression profiles of specific cell types; how theMiRNAs integrate into the existing gene regulatory networks; and how themiRNAs influence the evolution of 3'UTR of mammalian mRNAs.
Potential for neural regeneration after neurotoxic injury in the adult mammalian retina
Sotaro Ooto,Tadamichi Akagi,Ryoichiro Kageyama,Joe Akita,Michiko Mandai,Yoshihito Honda,Masayo Takahashi +6 more
TL;DR: This study observed that some Müller glial cells were stimulated to proliferate in response to a toxic injury and produce bipolar cells and rod photoreceptors, and demonstrated that retinal neurons regenerated even in adult mammalian retina after toxic injury.
Pax6 regulates gene expression in the vertebrate lens through miR-204.
Ohad Shaham,Karen Gueta,Eyal Mor,Pazit Oren-Giladi,Dina Grinberg,Qing Xie,Ales Cvekl,Noam Shomron,Noa Davis,Maya Keydar-Prizant,Shaul Raviv,Metsada Pasmanik-Chor,Rachel E. Bell,Carmit Levy,Raffaella Avellino,Sandro Banfi,Ivan Conte,Ruth Ashery-Padan +17 more
TL;DR: It is revealed that Trpm3 and the intronic microRNA gene miR-204 are co-regulated by Pax6 during eye development, which is a novel evolutionarily conserved mechanism by which Pax6 controls the down-regulation of multiple genes through direct up- regulation of miR -204.
Gene delivery to the retina: focus on non-viral approaches.
TL;DR: This review focuses on different non-viral vectors for gene delivery to the retina, the barriers that such delivery systems face and methods to overcome them.
The expression of glia maturation factors and the effect of glia maturation factor-γ on angiogenic sprouting in zebrafish
TL;DR: These findings provide the first in vivo evidence that GMFG is an important regulator for angiogenic sprouting during angiogenesis in zebrafish and suggest thatGMFG may act as a novel potential target for anti-angiogenesis therapy in clinical settings.