Open AccessJournal Article
IDENTIFICAÇÃO E ENSAIO DE ATIVAÇÃO BIOQUÍMICA DE CEPAS GRAS DE Saccharomyces cerevisiae COM POTENCIAL BIOTECNOLÓGICO
TL;DR: A pesquisa objetivou realizar a identificacao e ativacao bioquimica de cepa de S. cerevisiae com potencial biotecnologico exposta a condicoes de inviabilidade celular as mentioned in this paper.
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Abstract: O Saccharomyces cerevisiae e leveduras de importância biotecnologica associada a industrias alimenticias, sucroalcooleira pela participacao nos processos fermentativos. Esta pesquisa objetivou realizar a identificacao e ativacao bioquimica de cepa de S. cerevisiae com potencial biotecnologico exposta a condicoes de inviabilidade celular. A ativacao foi realizada por reidratacao em solucao salina de NaCl a 0,9% a 37o C ± 1o C por 5 min, seguida da agitacao em vortex por 5 min. Posteriormente, aliquotas de 100µl foram semeada em meio agar Sabouraud Dextrose (CAF) a 37o C. A analise da viabilidade foi realizada em microscopia, teste em azul de metileno, fermentacao e a identificacao com kit Fungifast da Elitech Microbio - Ref. 44430, apos 24 h. Os resultados confirmaram ser cepas de S. cerevisiae que, apos os ensaios, apresentando-se viaveis e com atividades fermentativas e peroxidase positiva. As tecnicas foram praticas, eficazes e podem contribuir com o aprimoramento da ativacao e identificacao de leveduras.
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