Human first-trimester fetal MSC express pluripotency markers and grow faster and have longer telomeres than adult MSC.
TL;DR: Compared with adult MSC, first‐trimester fetal tissues constitute a source of MSC with characteristics that appear advantageous for cell therapy, andfetal MSC were also more readily expandable and senesced later in culture than their adult counterparts.
read more
Abstract: The biological properties of stem cells are key to the success of cell therapy, for which MSC are promising candidates. Although most therapeutic applications to date have used adult bone marrow MSC, increasing evidence suggests that MSC from neonatal and mid-gestational fetal tissues are more plastic and grow faster. Fetal stem cells have been isolated earlier in development, from first-trimester blood and hemopoietic organs, raising the question of whether they are biologically closer to embryonic stem cells and thus have advantages over adult bone marrow MSC. In this study, we show that human first-trimester fetal blood, liver, and bone marrow MSC but not adult MSC express the pluripotency stem cell markers Oct-4, Nanog, Rex-1, SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81. In addition, fetal MSC, irrespective of source, had longer telomeres (p < .001), had greater telomerase activity (p < .01), and expressed more human telomerase reverse transcriptase (p < .01). Fetal MSC were also more readily expandable and senesced later in culture than their adult counterparts (p < .01). Compared with adult MSC, first-trimester fetal tissues constitute a source of MSC with characteristics that appear advantageous for cell therapy.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Reviewing and updating the major molecular markers for stem cells.
TL;DR: New and alternative markers for SCs are presented, along with microRNA profiles, for these cells, and these cells are mainly isolated based on the expression of CD34.
184
A consensus introduction to serum replacements and serum-free media for cellular therapies
Ohad Karnieli,Oryan Makler Friedner,Julie G. Allickson,Nan Zhang,Sunghoon Jung,D. Fiorentini,Eytan Abraham,Shannon Eaker,tan Kah Yong,Allan Chan,Sarah Griffiths,Amy K. Wehn,Steve Oh +12 more
TL;DR: The paper reviews the available serum replacements, main components, basic strategies for replacement of serum and suggests definitions, where there are no accepted definitions for most of these terms which leads to misleading's and misunderstandings.
177
Valproic Acid Confers Functional Pluripotency to Human Amniotic Fluid Stem Cells in a Transgene-free Approach
Dafni Moschidou,Sayandip Mukherjee,Michael P. Blundell,Katharina Drews,Gemma N. Jones,Hassan Abdulrazzak,Beata Nowakowska,Anju Phoolchund,Kenneth Lay,T Selvee Ramasamy,Mara Cananzi,Daniel Nettersheim,Mark H.F. Sullivan,Jennifer M. Frost,Gudrun E. Moore,Joris Vermeesch,Nicholas M. Fisk,Adrian J. Thrasher,Anthony Atala,James Adjaye,James Adjaye,Hubert Schorle,Paolo De Coppi,Pascale V. Guillot +23 more
TL;DR: It is shown that c-KIT+ human first-trimester amniotic fluid stem cells (AFSCs) can be fully reprogrammed to pluripotency without ectopic factors and are utilized for cell banking of patient-specific pluripotent cells for potential applications in allogeneic cellular replacement therapies, pharmaceutical screening, and disease modeling.
167
Stem cells derived from amniotic fluid: new potentials in regenerative medicine.
TL;DR: Amniotic fluid stem cells represent a novel class of pluripotent stem cells with intermediate characteristics between embryonic and adult stem cells, as they are able to differentiate into lineages representative of all three germ layers but do not form tumours when injected in vivo.
166
Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues
TL;DR: Foetal stem cells have been used in pre-clinical studies to treat a wide range of diseases such as skeletal dysplasia, diaphragmatic hernia and respiratory failure, white matter damage, renal pathologies as well as cancers.
156
References
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
22K
A simple salting out procedure for extracting DNA from human nucleated cells
TL;DR: A rapid, safe and inexpensive method was developed to simplify the deprotein-ization procedure that yielded quantities comparable to those obtained from phenol-chloroform extractions, rendering the entire process of RFLP analysis free of toxic materials.
21.6K
Mesenchymal stem cells
TL;DR: The study of mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self‐cell repair.
6.2K
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
6K
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.