Journal Article10.1126/SCIENCE.3576223
Human amnion membrane serves as a substratum for growing axons in vitro and in vivo
TL;DR: This amnion preparation can serve as a bridge to promote axonal regeneration in vivo in damaged adult brain by regenerating axons of cultured neurons along pathways of basement membrane that were immunoreactive with an antibody to laminin.
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Abstract: The epithelial cell layer of human amnion membrane can be removed while the basement membrane and stromal surfaces remain morphologically intact. Such a preparation has been used as a substratum for the in vitro culture of dissociated neurons. Embryonic motor neurons from chick ciliary ganglion attached to both surfaces but grew extensive neurites only on the basement membrane. On cross sections of rolled amnion membranes, regenerating axons of cultured neurons were guided along pathways of basement membrane that were immunoreactive with an antibody to laminin. In addition, when rolled amnion membranes were implanted into a lesion cavity between the rat septum and hippocampus, cholinergic neurons extended axons through the longitudinally oriented implant into the hippocampus. Thus, this amnion preparation can serve as a bridge to promote axonal regeneration in vivo in damaged adult brain.
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Citations
Poly lactic acid–caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves
Nurru Mligiliche,Yasuhiko Tabata,Masaaki Kitada,Katsuaki Endoh,Keiko Okamato,Etsuko Fujimoto,Chizuka Ide +6 more
TL;DR: Results indicate that the PLAC tube with a denatured muscle segment inside provided good conditions for nerve fiber regrowth, and the hindlimb muscle was innervated by motor nerves in all animals 5 months after implantation.
Nerve Growth Factor in Vivo Actions on Cholinergic Neurons in the Adult Rat CNS
Silvio Varon,Theo Hagg,H. Lee Vahlsing,Marston Manthorpe +3 more
- 01 Jan 1988
TL;DR: Neurite-Promoting Factors specifically stimulate the outgrowth of neuronal processes (“neuritis”) and may assist, but not substitute for, the required NTFs, while NeuronoTrophic Factors are special proteins controlling survival, growth and functional capabilities of selected populations of neurons.
In toto differentiation of human amniotic membrane towards the Schwann cell lineage
Asmita Banerjee,Sylvia Nürnberger,Simone Hennerbichler,Sabrina Riedl,Christina M.A.P. Schuh,Ara Hacobian,Andreas H. Teuschl,Johann Eibl,Heinz Redl,Susanne Wolbank +9 more
TL;DR: It is hypothesize that viable hAM without prior cell isolation can be differentiated towards the Schwann cell lineage to generate a possible alternative to commonly applied tissue engineering materials for nerve regeneration.
Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stromal/Stem Cell Seeding.
Haadia Tauseef,Kainat Ahmed,Asmat Salim,Omair A. Mohiuddin +3 more
TL;DR: Decellularized amniotic membrane, derived from discarded placental tissue, is converted into a bio-scaffold for regenerative medicine, supporting stem cell adhesion, growth, and proliferation, with adipose-derived stem cells seeded onto its extracellular matrix.
Anatomy and Pathology of the Placental Membranes
Kurt Benirschke,Peter Kaufmann +1 more
- 01 Jan 2000
TL;DR: The structure and function of the membranes have received considerable attention primarily because of an interest in the turnover of the water they contain and the composition of the various extracellular connective tissue components has come under scrutiny.
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Regeneration of the septohippocampal pathways in adult rats is promoted by utilizing embryonic hippocampal implants as bridges
TL;DR: Evidence is provided that axonal regeneration and reinnervation of a denervated target zone can be promoted by utilizing implants of embryonic CNS tissue to bridge a tissue defect between the target and the lesioned axonal stumps.
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TL;DR: The fluid surrounding the regenerating nerve was found to contain NPF activity for cultured ciliary ganglia neurons which markedly increased during the period of neurite growth into the chamber, consistent with a role of fibronectin in promoting cell migration.
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New method for preparing large surfaces of intact human basement membrane for tumor invasion studies
TL;DR: A new method has been developed to prepare large surfaces of continuous native human basement membrane for in vitro studies of tumor invasion and local dissolution of the lamina densa occurs at the region of cell attachment.
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