Journal Article10.1126/SCIENCE.2188365
HSP104 required for induced thermotolerance.
Yolanda Sánchez,Susan Lindquist +1 more
TL;DR: It is demonstrated that a particular heat shock protein plays a critical role in cell survival at extreme temperatures and is rescued with the wild-type gene.
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Abstract: A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.
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Citations
The cytoplasmic chaperone hsp104 is required for conformational repair of heat-denatured proteins in the yeast endoplasmic reticulum
TL;DR: It is shown that conformational repair of heat-damaged glycoproteins in the ER of living yeast cells required functional Hsp104, and that Hsp 104 appears to control conformational Repair of heat -damaged proteins even beyond the ER membrane.
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The role of catalase in hydrogen peroxide resistance in fission yeast Schizosaccharomyces pombe.
TL;DR: The results indicate that the catalase of S. pombe plays an important role in resistance to high concentrations of hydrogen peroxide but offers little in the way of protection from the hydrogenperoxide generated in small amounts under normal growth conditions.
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•Book
Molecular Biology and its Application to Medical Mycology
Bruno Maresca,George S. Kobayashi,Hideyo Yamaguchi +2 more
- 22 Jan 2012
TL;DR: Molecular Biology of Yeast, Heat Shock Response and Adaptation during Morphogenesis in Histoplasma capsulatum, and Imaging of the Yeast Killer Phenomenon.
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Do mitochondria regulate the heat-shock response in Saccharomyces cerevisiae ?
E. G. Rikhvanov,N. N. Varakina,T. M. Rusaleva,E. I. Rachenko,Dmitry A. Knorre,Victor K. Voinikov +5 more
TL;DR: The results obtained clearly indicate that a mild heat shock elicits a hyperpolarization of the inner mitochondrial membrane and such an event is one of several signals triggering the chain of reactions that activates the expression of the HSP104 gene and probably theexpression of other heat shock-regulated genes in S. cerevisiae.
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Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae.
TL;DR: It is shown that increased trehalose accumulation and increased heat resistance caused by overexpression of LRE1 are also the result of inhibition of Cbk1, revealing a novel control pathway for certain targets affected by PKA.
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References
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Robert S. Sikorski,Philip Hieter +1 more
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
The heat-shock proteins
Susan Lindquist,E A Craig +1 more
TL;DR: Roles moleculaires des proteines de choc thermique dans le fonctionnement des organismes a des temperatures normales et suite a des chocs thermiques; differents genes impliques.
5.3K
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange
TL;DR: A simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis, using the metachromatic stain acridine orange for visualization ofucleic acids in gels and which can be analyzed on the same slab gel.
2.4K
The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses
TL;DR: Results indicate that UBI4 is specifically required for the resistance of cells to stress, and that ubiquitin is an essential component of the stress response system.
886