Journal Article10.1126/SCIENCE.2188365
HSP104 required for induced thermotolerance.
Yolanda Sánchez,Susan Lindquist +1 more
TL;DR: It is demonstrated that a particular heat shock protein plays a critical role in cell survival at extreme temperatures and is rescued with the wild-type gene.
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Abstract: A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.
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Citations
The heat shock protein ClpB mediates the development of thermotolerance in the cyanobacterium Synechococcus sp. strain PCC 7942.
TL;DR: Results clearly show that induction of ClpB at high temperatures is vital for sustained thermotolerance in Synechococcus spp.
116
[PSI+]: An Epigenetic Modulator of Translation Termination Efficiency
Tricia R. Serio,Susan Lindquist +1 more
TL;DR: The series of experiments supporting the yeast prion hypothesis are reviewed and another look is provided at the 30 years of work preceding this theory in light of the current state of knowledge.
Prolyl isomerases in yeast.
TL;DR: The study of prolyl isomerases in S. cerevisiae has proven invaluable to understand the elusive functions of these proteins, and continues to provide new insights into their diverse cellular roles.
115
Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
Alexandrea N. Rizo,Alexandrea N. Rizo,JiaBei Lin,Stephanie N. Gates,Eric Tse,Stephen M. Bart,Laura M. Castellano,Frank DiMaio,James Shorter,Daniel R. Southworth +9 more
TL;DR: NBD conformations at the seam interface reveal how ATP hydrolysis-driven substrate disengagement and re-binding are precisely tuned to drive a directional, stepwise translocation cycle.
Leishmania major Hsp100 is required chiefly in the mammalian stage of the parasite.
TL;DR: The results suggest an important role for this parasite heat shock protein during the initial stages of a mammalian infection and overexpression of exogenous clpB gene copies can partly restore virulence to the gene replacement mutants.
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