Journal Article10.1126/SCIENCE.2188365
HSP104 required for induced thermotolerance.
Yolanda Sánchez,Susan Lindquist +1 more
TL;DR: It is demonstrated that a particular heat shock protein plays a critical role in cell survival at extreme temperatures and is rescued with the wild-type gene.
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Abstract: A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.
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Citations
Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation
Axel Mogk,Eva Kummer,Bernd Bukau +2 more
TL;DR: This work has shown that a bi-chaperone system composed of ATP-dependent Hsp70 and hexameric Hsp100 (ClpB/Hsp104) chaperones, which rescue aggregated proteins and provide thermotolerance to cells, is crucial for cell viability as permanently activated Hsp 100 variants are toxic.
Stationary-Phase Gene Expression in Saccharomyces cerevisiae During Wine Fermentation
TL;DR: In this paper, a haploid wine strain of Saccharomyces cerevisia was grown on synthetic or natural winery musts and the expression profiles of nine genes, including members of the HSP family, were analyzed.
170
The molecular chaperone Hsp104--a molecular machine for protein disaggregation.
TL;DR: Hsp104 is an ATP dependent molecular machine that-in cooperation with Hsp70 and Hsp40-extracts polypeptide chains from protein aggregates and facilitates their refolding, although the molecular details of this process are still poorly understood.
169
A new method for manipulating transgenes: engineering heat tolerance in a complex, multicellular organism.
TL;DR: It is shown that manipulating the expression of a single hsp can be sufficient to improve the stress tolerance of a complex multicellular organism, and also that the role of the 70 kD heat-shock protein, Hsp 70, in thermotolerance is investigated.
168
Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants
Tatsuhiko Kadowaki,Shaoping Chen,Midori Hitomi,Erica Y. Jacobs,Chino Kumagai,Shuang Liang,Roger Schneiter,David Rivers Singleton,Joanna Wisniewska,Alan M. Tartakoff +9 more
TL;DR: To understand the mechanisms of mRNA transport in eukaryotes, Saccharomyces cerevisiae temperature-sensitive mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature are isolated and classified into 16 complementation groups.
167
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