Journal Article10.1126/SCIENCE.2188365
HSP104 required for induced thermotolerance.
Yolanda Sánchez,Susan Lindquist +1 more
TL;DR: It is demonstrated that a particular heat shock protein plays a critical role in cell survival at extreme temperatures and is rescued with the wild-type gene.
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Abstract: A heat shock protein gene, HSP104, was isolated from Saccharomyces cerevisiae and a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.
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Citations
Patent
Recombinant prion-like proteins and materials comprising same
Susan Lindquist,Liming Li,Jiyan Ma,Jia-Jia Liu,Neal Sondheimer,Thomas Scheibel +5 more
- 09 Jun 2000
TL;DR: In this article, the authors present novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptic codes; host cells transformed or transfected with such polynuclear codes; and methods of making and using the foregoing.
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In memory of Susan Lindquist (1949–2016)
TL;DR: The current issue of Prion marks the 10-year anniversary since the journal has started, and unfortunately, Prion enters its second decade without one of the valuable members of its Editorial Board, Dr. Susan Lindquist, who died of cancer on October 27, 2016.
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The Effect of Sodium Azide on Basic and Induced Thermotolerance in Saccharomyces cerevisiae
E. I. Rachenko,E. G. Rikhvanov,N. N. Varakina,T. M. Rusaleva,G. B. Borovskii,Victor K. Voinikov +5 more
TL;DR: The protective effect of sodium azide on the thermotolerance of S. cerevisiae cells grown on glucose-containing medium is not related to Hsp104 functioning, and the mechanisms of basic and induced thermot tolerance differ considerably.
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Adaptive significance of polyploidy in brine shrimp (Artemia parthenogenetica)
Lei Zhang
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