Journal Article10.1021/JP807337F
Highly sensitive glucose biosensor based on one-pot biochemical preoxidation and electropolymerization of 2,5-dimercapto-1,3,4-thiadiazole in glucose oxidase-containing aqueous suspension.
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TL;DR: The electrochemical quartz crystal microbalance technique was used to investigate various electrode modification processes, which revealed that poly(DMcT) could be cathodically detached from the electrode surface, favorably enabling the electrochemical regeneration of the electrode substrate.
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Abstract: A novel and high-performance biosensing platform was prepared on the basis of one-pot biochemical preoxidation and electropolymerization of monomer (BPEM) for high-load and high-activity immobilization of enzymes. As representative materials here, 2,5-dimercapto-1,3,4-thiadiazole (DMcT) was used as the monomer, glucose oxidase (GOx) was used as the model enzyme, and enzymatically generated H2O2 (EG-H2O2) in the presence of glucose was used as the preoxidant. In the BPEM protocol, glucose was added to a pH 7.0 phosphate buffer suspension containing ultrasonically dispersed DMcT and GOx, in order to enzymatically generate H2O2, which preoxidized DMcT to DMcT oligomers/polymer (DMcTO) and thus led to the formation of DMcTO−GOx composites with a great deal of GOx entrapped; the composites were then co-electrodeposited with poly(DMcT) on a Au electrode. For comparison, the enzyme immobilization was also conducted by a preoxidation-free conventional electropolymerization protocol (CEP), as well as a chemical pr...
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Citations
Recent advances in electrochemical glucose biosensors: a review
TL;DR: In this paper, the authors reviewed principles, methods and recent developments in electrochemical glucose sensors and discussed some problems and bottlenecks in areas of nonenzymatic and enzymatic (glucose oxidase-based) amperometric glucose sensing.
Electrosynthesized polymers for biosensing.
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Multifunctional carbon nanotubes and their derived nano-constructs for enzyme immobilization – A paradigm shift in biocatalyst design
TL;DR: The current review spotlights recent advances in the immobilization of various enzymes on multifunctional CNTs and their derived nano-constructs and potential applications in different fields such as electrochemical biosensing, biodiesel production, environmental remediation and protection, and lactose bioconversion are discussed.
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Differential pulse anodic stripping voltammetric determination of Cd and Pb at a bismuth glassy carbon electrode modified with Nafion, poly(2,5-dimercapto-1,3,4-thiadiazole) and multiwalled carbon nanotubes
Xiuhui He,Zhaohong Su,Qingji Xie,Chao Chen,Yingchun Fu,Li Chen,Ying Liu,Ming Ma,Le Deng,Dilan Qin,Yueping Luo,Shouzhuo Yao +11 more
TL;DR: Differential pulse anodic stripping voltammetry was applied to detect Pb2+ and Cd2+ at a glassy carbon electrode modified with Nafion, poly(2,5-dimercapto-1,3,4-thiadiazole) (PDMcT), and multiwalled carbon nanotubes (MWCNTs) as discussed by the authors.
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A glucose biosensor based on chitosan–glucose oxidase–gold nanoparticles biocomposite formed by one-step electrodeposition
TL;DR: The combination of gold nanoparticles affinity and the promising feature of the biocomposite with the onestep nonmanual technique favor the sensitive determination of glucose with improved analytical capabilities.
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