Highly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis

TL;DR: It is found that seedlings exposed to microgravity had lower methylation on average than control plants grown on the ground, although certain genes related to methylation, transcription factors and hormones tended to be more methylated, which could help inform the design of plants optimized for growth in space.

TL;DR: This review focuses on the individual and combined roles of histone variants in transcriptional regulation within euchromatic and heterochromatic genome regions and highlights gaps in the understanding of plant variants at the molecular, cellular, and organismal levels.

TL;DR: Examination of genome-wide DNA methylation in partial and complete loss-of-function met1 mutants provides evidence that DNA sequence features such as density of CpGs and genomic repetitiveness can be used to predict susceptibility to epiallelic switching.

TL;DR: Genome Bisulfite Sequencing Analyser (GBSA) is presented, a free open-source software capable of analysing whole-genome bisulfite sequencing data with either a gene-centric or gene-independent focus, and its output can be easily integrated with other high-throughput sequencing data to elucidate the role of methylated intergenic regions in gene regulation.

TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.

TL;DR: A genomic sequencing method is reported that provides positive identification of 5-methylcytosine residues and yields strand-specific sequences of individual molecules in genomic DNA, which suggests that the high methylation level of single-copy sequences in sperm may be locally modulated by binding of protein factors in germ-line cells.

TL;DR: Application of an algorithm incorporating all eight characteristics associated with siRNA functionality significantly improves potent siRNA selection and highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies.

TL;DR: The biological significance of 5-methylcytosine was in doubt for many years, but is no longer, and it has become clear that programmed changes in methylation patterns are less important in the regulation of mammalian development than was previously believed.