Highly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis

TL;DR: It is shown that DME contributes to DNA demethylase activity and disease response in somatic tissues, and this rdd DME RNAi line has enhanced disease susceptibility to Fusarium oxysporum infection compared to the rdd triple mutant.

TL;DR: This research investigates the use of RNA sequencing datasets to address the isoform quantification problem and identifies several alternatively-spliced genes with switch-like, on/off expression properties, as well as a number of other genes that varied more subtly in isoform expression.

TL;DR: The broad distribution of published data sets represents a viable starting point for the molecular dissection of the blood-brain barrier and will further direct the discovery of novel mechanisms of blood- brain barrier formation and function.

TL;DR: In this article, hairpin RNA (hpRNA) constructs consisting of an inverted repeat (IR) of target promoters directly introduced into an expression cassette have been used to mediate de novo DNA methylation.

TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.

TL;DR: A genomic sequencing method is reported that provides positive identification of 5-methylcytosine residues and yields strand-specific sequences of individual molecules in genomic DNA, which suggests that the high methylation level of single-copy sequences in sperm may be locally modulated by binding of protein factors in germ-line cells.

TL;DR: Application of an algorithm incorporating all eight characteristics associated with siRNA functionality significantly improves potent siRNA selection and highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies.

TL;DR: The biological significance of 5-methylcytosine was in doubt for many years, but is no longer, and it has become clear that programmed changes in methylation patterns are less important in the regulation of mammalian development than was previously believed.