Highly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis

TL;DR: The present review combines multi-disciplinary data from mitochondria, development, epigenetics and tumorigenesis, which could provide novel insights for further research, especially for developmental disorders and cancers.

TL;DR: A critical review of recent spaceflight experiments is provided and ways in which future experiments can be designed to improve the value and applicability of the results generated are suggested.

TL;DR: It is shown that RMR1 is necessary for the accumulation of a majority of 24 nt small RNAs, including those derived from Long-Terminal Repeat (LTR) retrotransposons, the most common repetitive feature in the maize genome.

TL;DR: In this paper, the authors identified PKL, a gene required for developmental regulation in plants, as a factor promoting transcriptional silencing at the transgenic RD29A promoter, and showed that PKL is required for the accumulation of Pol V-dependent transcripts and for the positioning of pol V-stabilized nucleosomes at several tested loci.

TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.

TL;DR: A genomic sequencing method is reported that provides positive identification of 5-methylcytosine residues and yields strand-specific sequences of individual molecules in genomic DNA, which suggests that the high methylation level of single-copy sequences in sperm may be locally modulated by binding of protein factors in germ-line cells.

TL;DR: Application of an algorithm incorporating all eight characteristics associated with siRNA functionality significantly improves potent siRNA selection and highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies.

TL;DR: The biological significance of 5-methylcytosine was in doubt for many years, but is no longer, and it has become clear that programmed changes in methylation patterns are less important in the regulation of mammalian development than was previously believed.