High-sensitive protein sequence analysis

TL;DR: These are the essential techniques which facilitate protein purification and sequence analysis in the picomole scale, such as HPLC chromatography, blotting for direct amino acid analysis and N-terminal sequencing.

Abstract: The primary structure determina peptides has made rapid progress in the recent years and beca- me an increasingly important tool of basic and applied re- search in various areas of biochemistry, molecular biology, medicine and biotechnology. Direct amino acid sequence analy- sis is frequently applied in combination with mass spectro- metry or DNA-sequencing of the protein's gene. Summarized are the essential techniques which facilitate protein purification and sequence analysis in the picomole scale, such as HPLC chromatography, blotting for direct amino acid analysis and N-terminal sequencing. Modern sequencer design is outlined.