High-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris
Rongzeng Liu,Bing Zhao,Yanling Zhang,Junxiang Gu,Mingrong Yu,Houyan Song,Houyan Song,Min Yu,Wei Mo +8 more
TL;DR: The high purity and activity of the rhμPlg obtained here will likely prove to be a valuable tool for studies of its application in thrombotic diseases and vitreoretinopathies, as well as a solid foundation for its downstream application.
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Abstract: Background
Plasmin is a serine protease that plays a critical role in fibrinolysis, which is a process that prevents blood clots from growing and becoming problematic Recombinant human microplasminogen (rhμPlg) is a derivative of plasmin that solely consists of the catalytic domain of human plasmin and lacks the five kringle domains found in the native protein Developing an industrial production method that provides high yields of this protein with high purity, quality, and potency is critical for preclinical research
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Citations
Structure and activity of plasmin and other direct thrombolytic agents
Valery Novokhatny
- 01 Jan 2008
TL;DR: Talecris Biotherapeutics has developed a methodology to prepare the plasmin product (Human) TAL-05-00018 that has shown promise in in vitro and pre-clinical studies, and in early clinical trials, demonstrating a dose-dependant reduction in clot weight that compares favorably to that seen with tPA.
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The N-terminal-truncated recombinant fibrin(ogen)olytic serine protease improves its functional property, demonstrates in vivo anticoagulant and plasma defibrinogenation activity as well as pre-clinical safety in rodent model.
Bandana Bora,Debananda Gogoi,Debabrata Tripathy,Sillarine Kurkalang,Sheetal Ramani,Anupam Chatterjee,Ashis K. Mukherjee +6 more
TL;DR: It is proposed that [Bacifrinase (ΔN24], at a dose of 2mg/kg, may serve as prototype for the development of potent drug to prevent hyperfibrinogenemia related disorders.
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In vitro fibrinolysis and antithrombosis characterizations of novel recombinant microplasminogen with RGD and GPRP peptides.
TL;DR: Two recombinant μPlg variants containing tripeptide Arg-Gly-Asp (RGD) and tetrapeptide Gly-Pro-Arg-Pro (GPRP) by site-directed mutagenesis are presented, suggesting the anti-thrombosis properties of these two μ Plg derivatives.
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Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris.
Roghayyeh Baghban,Safar Farajnia,Younes Ghasemi,Reyhaneh Hoseinpoor,Azam Safary,Mojtaba Mortazavi,Nosratollah Zarghami +6 more
TL;DR: The results indicated that combined mutations at the ocriplasmin sequence were more effective compared with single mutations, and represent valuable tools for the investigation of therapeutic strategies aiming at the non-surgical resolution of vitreomacular adhesion.
Active Expression of Human Tissue Plasminogen Activator (t-PA) c-DNA from Pulmonary Metastases in the Methylotrophic Yeast Pichia Pastoris KM71H Strain
Amir Hossein Mohseni,Mohammad Soleimani,Mohammad Soleimani,Keivan Majidzadeh-A,Keivan Majidzadeh-A,Sedigheh Taghinezhad-S,Hossein Keyvani +6 more
TL;DR: Evaluation of t-PA expression in Pichia pastoris KM71H strains found it to be an appropriate strain for production of active recombinant protein and proteolytic activity and biological function of the expressed recombinant t- PA.
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References
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Plasminogen deficiency: PLASMINOGEN DEFICIENCY
R. Mehta,A. D. Shapiro +1 more
TL;DR: It appears that hypoplasminogenaemia (type I deficiency) is the type most associated with pseudomembrane disease, and a variety of genetic mutations has been identified recently and is reported to lead to these disorders.
186
Plasminogen has a broad extrahepatic distribution.
Lu Zhang,Dietmar Seiffert,Bruce Fowler,George R Jenkins,Therese C. Thinnes,David J. Loskutoff,Robert J. Parmer,Lindsey A. Miles +7 more
TL;DR: The results suggest that the plasminogen gene is expressed broadly in extrahepatic tissues, and tissues separated by local anatomic barriers as well as tissues accessible to circulating plAsminogen have the capacity to provide local sources of plasmineogen.
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