Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis.
Thomas Friedrich,Vicky Oberkofler,Inês Trindade,Simone Altmann,Simone Altmann,Krzysztof Brzezinka,Jörn Lämke,Michal Gorka,Christian Kappel,Ewelina M. Sokolowska,Aleksandra Skirycz,Alexander Graf,Isabel Bäurle +12 more
TL;DR: In this article, it was shown that the FORGETTER3/HEAT SHOCK TRANSCRIPTION FACTOR A3 (FGT3/HSFA3) is specifically required for physiological heat stress memory and maintaining high memory-gene expression during the days following a moderate heat stress exposure.
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Abstract: Adaptive plasticity in stress responses is a key element of plant survival strategies. For instance, moderate heat stress (HS) primes a plant to acquire thermotolerance, which allows subsequent survival of more severe HS conditions. Acquired thermotolerance is actively maintained over several days (HS memory) and involves the sustained induction of memory-related genes. Here we show that FORGETTER3/ HEAT SHOCK TRANSCRIPTION FACTOR A3 (FGT3/HSFA3) is specifically required for physiological HS memory and maintaining high memory-gene expression during the days following a HS exposure. HSFA3 mediates HS memory by direct transcriptional activation of memory-related genes after return to normal growth temperatures. HSFA3 binds HSFA2, and in vivo both proteins form heteromeric complexes with additional HSFs. Our results indicate that only complexes containing both HSFA2 and HSFA3 efficiently promote transcriptional memory by positively influencing histone H3 lysine 4 (H3K4) hyper-methylation. In summary, our work defines the major HSF complex controlling transcriptional memory and elucidates the in vivo dynamics of HSF complexes during somatic stress memory.
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Citations
Transcriptome and coexpression network analysis reveals properties properties and candidate genes associated with grape (Vitis vinifera L.) heat tolerance
Jiuyun Wu,Fuchun Zhang,Guohong Liu,Riziwangguli Abudureheman,Shijian Bai,Xinyu Wu,Chuan Zhang,Yaning Ma,Xiping Wang,Qian Zha,H.X. Zhong +10 more
TL;DR: This study observed the phenotypes and cellular structures of four grape varieties in the naturally high-temperature environment of Turpan to provide a theoretical foundation for further understanding the molecular mechanisms of grape heat tolerance and offer new gene resources for studying heat tolerance in grapes.
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High‐throughput identification of novel heat tolerance genes via genome‐wide pooled mutant screens in the model green alga <i>Chlamydomonas reinhardtii</i>
TL;DR: In this article , the authors used the insertional, indexed, genome-saturating mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii to perform genome-wide, quantitative, pooled screens under moderate (35°C) or acute (40°C), high temperatures with or without organic carbon sources.
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Memory of plants: present understanding
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TL;DR: Through this commentary, an understanding of the recent developments in area of plant memory and the molecular mechanisms involved in propagation of memory in plants is attempted.
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High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green Alga Chlamydomonas reinhardtii
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TL;DR: This work utilized the insertional mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii to perform genome-wide, quantitative, pooled screens under moderate or acute high temperatures with or without organic carbon and identified putative heat tolerance genes (HTGs).
BcWRKY22 Activates BcCAT2 to Enhance Catalase (CAT) Activity and Reduce Hydrogen Peroxide (H2O2) Accumulation, Promoting Thermotolerance in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)
Haiyan Wang,Zhanyuan Gao,Xiaoshan Chen,Entong Li,Ying Li,X. Hu +5 more
TL;DR: Findings suggest that BcWRKY22 may serve as a novel regulator of the heat stress response in non-heading Chinese cabbage, actively contributing to the establishment of thermal tolerance by upregulating catalase (CAT) activity and downregulating H2O2 accumulation via BcCAT2 expression.
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