Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.
Albert W. Tam,Matthew M. Smith,Martha E. Guerra,Chiao Chain Huang,Daniel W. Bradley,Kirk E. Fry,Gregory R. Reyes +6 more
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TL;DR: Findings on the genetic organization and expression strategy of HEV suggest that it is the prototype human pathogen for a new class of RNA virus or perhaps a separate genus within the Caliciviridae family.
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About: This article is published in Virology. The article was published on 01 Nov 1991. and is currently open access. The article focuses on the topics: RNA virus & Hepatitis E virus.
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Citations
A novel virus in swine is closely related to the human hepatitis E virus
Xiang-Jin Meng,Robert H. Purcell,Patrick G. Halbur,James R. Lehman,Dale M. Webb,Tatiana S. Tsareva,Joseph S. Haynes,Brad J. Thacker,Suzanne U. Emerson +8 more
TL;DR: The discovery of swine HEV not only has implications for HEV vaccine development, diagnosis, and biology, but also raises a potential public health concern for zoonosis or xenozoonosis following xenotransplantation with pig organs.
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Evolution and Taxonomy of Positive-Strand RNA Viruses: Implications of Comparative Analysis of Amino Acid Sequences
TL;DR: It is hypothesized that all positive-strand RNA viruses and some related double-stranded RNA viruses could have evolved from a common ancestor virus that contained genes for RNA-dependent RNA polymerase, a chymotrypsin-related protease that also functioned as the capsid protein, and possibly an RNA helicase.
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A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus.
Narayanan Jothikumar,Narayanan Jothikumar,Theresa L. Cromeans,Betty H. Robertson,Xiang-Jin Meng,Vincent R. Hill +5 more
TL;DR: The TaqMan assay detected as few as four genome equivalent (GE) copies of HEV plasmid DNA and detected as low as 0.12 50% pig infectious dose (PID50) of swine HEV in the real-time RT-PCR assay.
819
Hepatitis E: an emerging awareness of an old disease.
TL;DR: Although hepatitis E was recognized as a new disease in 1980, the virus was first visualized in 1983 and its genome was cloned and characterized in 1991, the disease is probably ancient but not recognized until modern times.
709
Hepatitis E: an emerging infection in developed countries
TL;DR: Patients with unexplained hepatitis should be tested for hepatitis E, whatever their age or travel history, and the source and route of infection remain uncertain, but it might be a porcine zoonosis.
696
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Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
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Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
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An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis
George Kuo,Q L Choo,Harvey J. Alter,G.L. Gitnick,Allan G. Redeker,Robert H. Purcell,Tatsuo Miyamura,Jules L. Dienstag,M.J. Alter,Cladd E. Stevens,Gary E. Tegtmeier,Ferruccio Bonino,Massimo Colombo,W.-S. Lee,C. Kuo,K. Berger,Jeffrey R. Shuster,Lacy Rasco Overby,Daniel W. Bradley,Michael Houghton +19 more
TL;DR: Assays of ten blood transfusions in the United States that resulted in chronic NANBH revealed that there was at least one positive blood donor in nine of these cases and that all ten recipients seroconverted during their illnesses.
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