Hemagglutination test for the diagnosis of human neurocysticercosis: development of a stable reagent using homologous and heterologous antigens.
Andréia P. Ferreira,Adelaide José Vaz,Paulo M. Nakamura,Álvaro Toshiaki Sasaki,Antônio Walter Ferreira,José Antonio Livramento +5 more
TL;DR: The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis.
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Abstract: A hemagglutination (HA) test was standardized using formalin- and tannin-treated gander red blood cells sensitized with a total salt extract of C. cellulosae (HA-Cc) and an antigenic extract of Cysticercus longicollis (HA-Cl) vesicular fluid. A total of 61 cerebrospinal fluid (CSF) samples were assayed, 41 from patients with neurocysticercosis and 20 from a control group which were, respectively, reactive and non-reactive to ELISA using C. cellulosae. The CSF samples from the control group did not react and 35 (85.4%) and 34 (82.9%) CSF samples from patients were reactive to the HA-Cc and HA-Cl tests, respectively. The reagents ready for use were stable up to 6 months when stored at 4 degrees C in 50% glycerol. The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis.
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Human cysticercosis and Indian scenario: a review
TL;DR: Literature search was done for this review with special emphasis on Indian studies to create awareness about the disease in India, since cysticercosis is preventable and potentially eradicable.
Immunodiagnostic tools for human and porcine cysticercosis.
TL;DR: The development of improved immunodiagnostic tools has contributed to the authors' knowledge on the importance of taeniosis/cysticercosis by enabling sero-epidemiological surveys and community-based studies to be carried out and the potential use of immunodiognostic tools to identify cases of neurocysticERCosis in man is subject to debate.
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Specific Taenia crassiceps and Taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples.
Ednéia Casagranda Bueno,Adelaide José Vaz,Luís dos Ramos Machado,José Antonio Livramento,Sílvia Regina Mielle +4 more
TL;DR: The use of Tcra ELISA is suggested for the study of serum and confirmation of the results for sera positive by an immunoblotting analysis in which specific peptides are detected.
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Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens
TL;DR: The search for antibodies revealed the presence of IgG > IgA > IgE in CSF, serum and saliva samples, with IgG being present in all phases of the disease, while IgA/IgE were more frequent in the inactive form.
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Detection of IgG in cerebrospinal fluid for diagnosis of neurocysticercosis: evaluation of saline and SDS extracts from Taenia solium and Taenia crassiceps metacestodes by ELISA and immunoblot assay
Ivanildes Solange da Costa Barcelos,José Roberto Mineo,Deise A. O. Silva,Marcelo Simão Ferreira,Leandro Pajuaba de Moura,Germano Francisco Biondi,Julia Maria Costa-Cruz +6 more
TL;DR: Overall the homologous antigenic extracts showed higher sensitivity than the heterologous extracts in the diagnosis of NC in CSF samples, which are recognized by IgG antibodies inCSF samples.
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References
•Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
318.1K
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
256.9K
•Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
203K
•Journal Article
Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium.
Carlos Larralde,Julio Sotelo,R. M. Montoya,G. Palencia,A. Padilla,Tzipe Govezensky,M. L. Diaz,Edda Sciutto +7 more
TL;DR: This manageable murine model of experimental cysticercosis solved the problem of antigen supply for clinical and epidemiological applications, and it provided an immediate means of abundant production of antigens for the wide distribution and standardization of immunodiagnostic tests for cystsercosis.
105
Cysticercosis of the central nervous system and cerebrospinal fluid immunodiagnosis of 1573 patients in 63 years (1929-1992)
TL;DR: Investigation of the incidence of neurocysticercosis in a region of Brazil finds that incidence continues expressively high throughout the 50 years covered by this study.