Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
read more
Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Rapid-cycling Brassica species: anther culture potential of B. campestris L. and B. napus L.
TL;DR: A study was made of the production of embryoids by cultured anthers of rapid-cycling populations of Brassica campestris L. and Brassica napus using a solidifed Brassica anther culture medium of Keller & Armstrong (K).
8
•Dissertation
Studies on micropropagation, cellular behavior, photosynthetic and biological activities of red clover (Trifolium pratense L.) / Arash Khorasani Esmaeili
Esmaeili Arash Khorasani
- 01 Jun 2016
TL;DR: In this paper, the authors investigated the effect of the solvent on cytotoxic activity of extracts of Trifolium pratense L. were tested for their antioxidant activities, using different extraction solvents and different antioxidant assays.
8
Tissue Culture Media—Composition and Preparation
Kenneth C. Torres
- 01 Jan 1989
TL;DR: The historical development of plant tissue culture media is described in numerous review articles and books and will not be discussed herein.
8
Prospects for the Use of Plant Cell Culture as Alternatives to Produce Secondary Metabolites
Hera Nadeem,Faheem Ahmad +1 more
- 01 Jan 2019
TL;DR: The aim of this chapter is to highlight the prospects of plant cell culture to produce secondary metabolites, and provides an overview on the important approaches used for the secondary metabolite production and their improvement strategies.
8
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
146
Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
85
An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
52