Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
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Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
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An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
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