Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
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Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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Citations
Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae).
Jorge Canhoto,Gil S. Cruz +1 more
TL;DR: Anthers of Feijoa sellowiana Berg (feijoa) produced pollen callus when cultured in Murashige and Skoog medium containing 2,4-dichlorophenoxyacetic acid and benzyladenine or in nurse cultures, and leaf-like structures could be obtained in regeneration media containing combinations of gibberellic acid and Benzylenine.
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Haploid plant regeneration from unpollinated ovule cultures of niger (Guizotia abyssinica (L. f.) Cass.)
J. G. Bhat,H. N. Murthy +1 more
TL;DR: Cytological preparation of root tips and chloroplast counts in the guard cells of leaf stomata of regenerated plants confirmed their haploid nature.
11
Investigating the most effective factors in the embryo rescue technique for use with ‘Flame Seedless’ grapevine (Vitis vinifera)
TL;DR: The results showed that using Nitsch and Nitsch medium rather than Emershad and Ramming medium for double-phase rather than solid phase media produced better developed embryos and germinated embryos in seedless grapevine cultivar ‘Flame Seedless’.
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Responses of callus from zygotal and microsporal tobacco (nicotiana tabacum l.) to various combinations of indole acetic acid and kinetin
TL;DR: Calluses from zygotal and microsporal plants of Nicotiana tabacum and Maryland Mammoth exhibited similar growth and morphogenetic responses to culture on twelve media consisting of a basal medium and all combinations of IAA and kinetin.
11
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
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Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
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An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
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