Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
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Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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Citations
Monoterpene Indole Alkaloids ( Catharanthus Alkaloids)
Vincenzo DeLuca,Wolfgang G. W. Kurz +1 more
- 01 Jan 1988
TL;DR: This chapter provides an overview on the production of indole alkaloids by Catharanthus plant cell cultures, and gives special attention to the enzymatic regulation of synthesis.
13
Anther culture of fragaria ananassa : environmental factors and medium components affecting microspore divisions and callus production
TL;DR: Of the growth regulators investigated, α-naphthaleneacetic acid combined with kinetin produced the highest frequency of calluses, and addition of L-glutamine to the basal medium increased frequency of microspore divisions and callus production in two out of three cultivars tested.
13
In-vitro induction of vegetative buds by benz(a)anthracene in tobacco callus.
TL;DR: Benz(a)anthracene induced vegetative buds on callus derived from stem tissue of haploid plants of Nicotiana tabacum a response similar to that obtained by a combination of kinetin and IAA.
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Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos.
Yosvanis Acanda,Yosvanis Acanda,Óscar Martínez,María Jesús Prado,María Victoria González,Manuel Rey +5 more
TL;DR: The results demonstrated the involvement of ABA metabolism in the control of the maturation of grapevine somatic embryos cultured over a semipermeable membrane and two important control points for their endogenous ABA levels.
Soybean androgenesis I: identification of pyramidal stressors in anther cultures that sustain cell divisions and putative embryo formation from isolated microspore cultures
Martina Garda,Brett Hale,Naina Rao,Morgan Lowe,Megan Bright,Shayn Goodling,Gregory C. Phillips,Gregory C. Phillips +7 more
TL;DR: The results across genotypes Jack, Thorne, Williams 82 and IAS-5 indicated that androgenesis can be stimulated up to 9 to 12% induction frequency by the use of 10°C day/8°C night for 3 d then 4°C overnight dark pretreatment of donor plants, and a series of incubation temperatures from 11°C to 18°Cs to 25°C, and nitrogen starvation medium.
13
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
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Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
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An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
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