Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
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Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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Citations
Pseudogamic production of dihaploids and monoploids in Solanum tuberosum and some related species
E.W.M. van Breukelen
- 01 Jan 1981
TL;DR: Attempts were made to maximize frequencies of dihaploids from Solanum tuberosum, obtained through pseudogamy after pollination with S.phureja using several S. phureja genotypes as pollinator, and the 2n-pollen but the n- pollen proved to be instrumental in di Haploid induction.
Interspecific hybridization between Nicotiana repanda Willd. and N. tabacum L. through the pollen irradiation technique and the egg cell irradiation technique.
TL;DR: It appears that it may be possible to overcome hybrid inviability to a certain extent using both the pollen irradiation technique and the egg cell irradiated technique, i.e., gamma-ray irradiation to pollen or to egg cells (in ovules) before pollination and in vitro culture of fertilized ovules.
16
Ein Standardnährmedium für die Anzucht von Kalluskulturen einiger Arzneipflanzen
TL;DR: A new standard medium for tissue cultures of medical plants has been developed, on which callus growth was tested either by tissue cultures in light and dark or by suspension cultures inlight.
16
Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii
Ives Vanstechelman,Tom Eeckhaut,Johan Van Huylenbroeck,Marie-Christine Van Labeke,Marie-Christine Van Labeke +4 more
TL;DR: The necessity of microscopic techniques for a thorough characterization of mixoploids and the potential of the combination of these techniques with flow cytometry to unravel the exact effect of mitosis inhibitors on monocot plants are demonstrated.
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Species with Haploid or Doubled Haploid Protocols.
TL;DR: In this paper, a list of species (and few interspecific hybrids) where haploids and/or doubled haploids have been published, including the method by which they were obtained and the corresponding references, is presented.
16
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
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Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
85
An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
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