Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
read more
Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
•Journal Article
Encapsulated somatic embryos of grape ( Vitis vinifera L.): An efficient way for storage and propagation of pathogen-free plant material
TL;DR: Cotyledonary-stage somatic embryos originating from leaf explants of grape ( Vitis vinifera L.) cv were encapsulated individually in 2 % alginate gel and normally developed plantlets regenerated from ESEs were successfully adapted to soil.
Micropropagation and molecular characterization of Thymus sibthorpii Benth. (Lamiaceae), an aromatic-medicinal thyme with ornamental value and conservation concern
Georgios Tsoktouridis,Nikos Krigas,Virginia Sarropoulou,S. Kampouropoulou,Katerina Papanastasi,Katerina Grigoriadou,Georgios Menexes,Eleni Maloupa +7 more
TL;DR: An efficient and reliable protocol for in vitro clonal propagation of T. sibthorpii from nodes and meristem tip explants was developed and shoot proliferation succeeded on a new basal medium (BB) without plant growth regulators, as prior experiments with 6-benzyladenine generated hyperhydricity.
17
Effect of red-, far-red- and blue-light-emitting diodes on in vitro growth of Ficus benjamina
Stefaan Werbrouck,H. Buyle,Danny Geelen,M. C. van Labeke +3 more
- 01 Oct 2012
TL;DR: It is shown that monochromatic blue, red and far-red and their combinations are suitable to manipulate the number of shoots, shoot length, shoot/callus weight ratio and leaf length/width ratio in Ficus benjamina.
17
Use of morphometric parameters for tracking ovule and microspore evolution in grapevine ( Vitis vinifera L., cv. “Aragonez”) and evaluation of their potential to improve in vitro somatic embryogenesis efficiency from gametophyte tissues
TL;DR: A correlation between micro- and macrosporogenesis evolutionary stages was positively established, allowing the use of morphometric parameters for tracking ovule evolution as well.
•Dissertation
Ploidy breeding and interspecific hybridization in Spathiphyllum and woody ornamentals
Tom Eeckhaut
- 01 Jan 2003
17
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
146
Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
85
An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
52