Journal Article10.1126/SCIENCE.163.3862.85
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
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Abstract: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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Citations
Strawberries ( Fragaria spp. and Hybrids)
F. Jungnickel
- 01 Jan 1988
TL;DR: Strawberries pioneered large-scale application of in vitro techniques in pomology and the scientific literature reflects all the ethusiasm and disillusionment, successes and failures, advantages and disadvantages, or finally, uses and misuses which accompany the introduction of a fundamental new method into practice.
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Maximizing seedling and root tuber production in Polygonum multiflorum for use in ethnomedicine
TL;DR: A standard protocol for the in vitro culture of Polygonum multiflorum to rapidly produce seedlings and promote the enlargement of its medicinal root tubers is developed, providing a basis for large-scale in vitro propagation of P. multiflorUM as a resource for traditional Chinese medicine.
18
The cytology of plantlets derived from cultured anthers of nicotiana sylvestris
Jen A. McComb,Arthur J. McComb +1 more
TL;DR: In microspores containing globular embryoids 0, 1 or 2 nuclei derived from the generative nucleus could be recognised; these, however, contributed few if any viable cells to the developing plantlets.
18
Imazalil enhances the shoot-inducing effect of benzyladenine in Spathiphyllum-floribundum Schott.
Spo Werbrouck,Pierre Debergh +1 more
TL;DR: In Spathiphyllum floribundum ‘Petite’ Schott shoot induction by benzyladenine (BA) was enhanced dramatically by adding the imidazole fungicide imazalil to the medium, and the number of shoots increased, and finally their size was reduced to a small meristematic dome.
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Chromosomal heterogeneity of Arabidopsis thaliana anther callus, regenerated shoots and plants
D.E. Keathley,R.L. Scholl +1 more
TL;DR: The dissimilarity of chromosome numbers of different groups of cells of the same callus suggests that the chromosomal heterogeneity arises during callus growth.
18
References
Ammonium Requirement for Embryogenesis in vitro
TL;DR: It is shown that undifferentiated pro-embryos, sieved from callus, will continue to grow in a disorganized non-polar fashion if they are exposed to a concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), and that it is possible to establish a crude control over the process of embryogenesis by suitable alterations of the 2,3-D concentration.
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Differentiation of embryoids from developing germ cells in anther culture of tobacco
Kazuo Nakata,Masao Tanaka +1 more
TL;DR: 1. 発育中のタバコの葯を組織培 養し, 培養中における花粉の形態変化を観察すると, 萎縮するもの, 登芽するがの, 多量のでんぷ
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An Androgenic Nicotiana Haploid
TL;DR: The androgenic haploid which developed in one of the ovules of the aberrant plant of N. Tabacum appears to be the first instance of such development in angiosperms where cytological proof of origin can be offered.
59
Interspecific Hybridization in Nicotiana. X. Haploid and Diploid Merogony
R. E. Clausen,W. E. Lammerts +1 more
TL;DR: No detectable influence of maternal cytoplasm was discernible in the above described instances and it is suggested that the occasional pure sylvestris plants obtained in backcrosses of F1 syLvestris-tabacum [Female] x syl Vestris [male] may arise from diploid merogony.
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